大葱叶片推陈出新中细胞内含物的再分配  

REDISTRIBUTION OF CELL CONTENTS BETWEEN DECLINING OLD AND GROWING YOUNG LEAVES IN SPRING ONION

作　　者：孙朝煜[1] 张蜀秋[1] 娄成后[1] 

机构地区：[1]植物生理学与生物化学国家重点实验室中国农业大学生物学院,北京100094

出　　处：《实验生物学报》2003年第1期13-17,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家重点基础研究专项经费资助(G1999011700)

摘　　要：大葱储藏过程中,新生叶片长出所需的营养来自衰老叶片储藏物质的再分配。电镜观察表明大葱叶鞘细胞结构变化表现出典型的编程性死亡特征;部分解体的原生质组分可能以囊泡迁移方式参与运输;酶定位显示在衰退叶鞘细胞的质膜、细胞壁和胞间连丝上有较高ATPase和APase活性,为该运输过程是依赖能量的主动运输补充了证据。During storage of a spring onion (Allium fistulosa var. dacong), the plant is unable to assimi late external resource, nutrient requirement of the new bud is entirely drawn from the withering leaf where both food reserves and protoplasmic constituents (i. e. organic complexes containing the essential mineral elements) are to be mobilized. Evidences were given to show that the withdrawal process of cell contents from the old part for establishing the new growth should be considered as an alternative mode or a supplementary mea sure of normal phloem transport of leaf photosynthates.Removal of cell contents from the declining old part to the developing new growth is of frequent occurrence in higher plants. In the present case, it followed the process of programmed cell death (PCD), That was examined and shown by electron microscopy and biochemical analysis. There appeared shrinkage of cell volume, dissolution of cell constitution and degradation of DNA. The present investigation has also shown that the with drawal of cell contents from the declining leaf sheath of spring onion can be wholly exhaustive. It may be carried out by active movement of the partially degraded protoplastllic constituents in forms of fragments and vesicles across the tissue, until hardly anything in the cellulose framework of the dry papery sheath, except a few crystals of Ca2+ . Presence of high ATPase and APase activities along the transport path also supports this view.

关 键 词：大葱叶片 细胞内含物 细胞编程性死亡 韧皮部运输 ATPASE APase 

分 类 号：Q945[生物学—植物学]