阿霉素及其铁配合物介导牛血清白蛋白氧化损伤  

Adriamycin or Adriamycin-Fe^(3+) Induced Bovine Serum Albumin Damage

作　　者：黄波[1] 邹国林[1] 金德龙 李海成[1] 

机构地区：[1]武汉大学生命科学学院,武汉430072

出　　处：《生物化学与生物物理学报》2003年第3期301-305,共5页

基　　金：国家自然科学基金资助项目 (No .39770 2 0 0 )~~

摘　　要：应用ESR波谱法研究了阿霉素 (ADM)及其铁 (Ⅲ )配合物 (ADM Fe3+)介导氧自由基产生的情况 ,结果表明ADM Fe3+介导产生氧自由基的能力比ADM更强。将ADM及ADM Fe3+分别作用于牛血清白蛋白 ,用分光光度法、聚丙烯酰胺凝胶电泳、荧光法检测蛋白质的损伤情况 ,发现ADM及ADM Fe3+均可通过氧化修饰肽链上的氨基酸残基来损伤蛋白质 ,且ADM Fe3+的作用更强。氧自由基清除剂对这种损伤有抑制作用 。ESR spectroscopy was used to investigate the production of reactive oxygen radicals in adriamycin (ADM) and its iron complex. The results showed that ADM-Fe 3+ induced the production of oxygen radicals more efficiently than ADM. The damage of BSA mediated by ADM or ADM-Fe 3+ was investigated by measuring the increase of reactive carbonyl content and the decrease of the fluorescence intensity of the Trp residue. It was shown that the damage of bovine serum albumin (BSA) was dependent on the time and on the concentration of ADM or ADM-Fe 3+. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that BSA was not cross-linked nor fragmented by the effect of ADM or ADM-Fe 3+. It was also found that oxygen radical scavengers could inhibit the damage of BSA induced by ADM or ADM-Fe 3+, suggesting that ADM or ADM-Fe 3+ induced protein damage via the oxygen radicals produced in the reaction.

关 键 词：阿霉素 铁配合物 介导 牛血清白蛋白 氧化损伤 氧自由基 

分 类 号：R363[医药卫生—病理学]