hTERT cDNA片段的克隆及其单克隆抗体与喉癌的发病机制(英文)  

Cloning of hTERT cDNA Fragment and Application of Anti-hTERT Monoclonal Antibody in Mechanism of Laryngeal Carcinogenesis

作　　者：邱广斌[1] 贺光[1] 宫立国[1] 赵震[1] 潘子民[2] 唐玉钗[3] 孙开来[1] 

机构地区：[1]中国医科大学医学遗传学教研室,沈阳110001 [2]中国医科大学第一临床学院耳鼻喉科,沈阳110001 [3]南京军区福州总医院全军检验中心,福州350025

出　　处：《Acta Genetica Sinica》2003年第2期109-113,共5页

基　　金：国家自然科学基金 (3 0 1710 0 8);卫生部科学研究基金 (981198)项目资助~~

摘　　要：端粒酶的激活及其调控机制至今仍不清楚。为研究喉癌发生中端粒酶表达规律及激活的可能机制 ,我们克隆了hTERTcDNA片段并制备了抗hTERT单克隆抗体。应用此抗体对喉癌组织进行了免疫组化检测 ,发现喉癌分化程度降低与癌组织中hTERT阳性细胞率增高有关 ;而c Myc表达与hTERT表达呈明显正相关 ,提示c Myc可能对喉癌发生过程中端粒酶的激活起着重要作用。这些研究表明 ,喉癌的发生可能是由于c Myc的过度表达使端粒酶表达上调 ,从而使喉鳞状上皮细胞达到永生化 ,这一机制不仅存在于喉癌发生的早期 。The mechanism of telomerase activation is still not clear till now.In order to understand the expressional mode of telomerase and the mechanism of telomerase activation in laryngeal carcinogenesis,we cloned a fragment of hTERT cDNA and prepared a monoclonal antibody against hTERT.We performed immuno histochemical staining in laryngeal cancer tissues using this antibody.We found that the frequencies of hTERT positive cells were positively correlated with undifferentiation of cancer tissues and that the expression of hTERT was positively correlated with levels of c Myc,which suggested that c Myc might play an important role in activation of telomerase.These results revealed that overexpression of c Myc upregulated telomerase,which in turn resulted in immortalization of laryngeal squamous cells,and this mechanism existed not only in the initiation of laryngeal carcinogenesis but also in the whole process of cancer development.

关 键 词：hTERT CDNA片段 克隆 单克隆抗体 喉癌 发病机制 端粒酶 免疫组化 

分 类 号：R739.63[医药卫生—肿瘤]