弱酸树脂D113对S-腺苷-L-蛋氨酸的交换行为及分离应用研究  

STUDY ON THE ION-EXCHANGE OF S-ADENOSYL-L-METHIONINE ON D113 WEAK ACID RESIN AND ITS APPLICATION ON SEPARATING S-ADENOSYL-L-METHIONINE FROM YEAST CELLS

作　　者：刘惠[1] 林建平[1] 岑沛霖[1] 

机构地区：[1]浙江大学(玉泉)材化学院生物工程研究所,杭州310027

出　　处：《离子交换与吸附》2003年第2期104-110,共7页Ion Exchange and Adsorption

摘　　要：考察了S-腺苷-L-蛋氨酸 (S-adenosyl-L-methionine, SAM) 在弱酸树脂争光D113上的静态吸附及分离行为,利用Langmuir等温式对平衡等温线进行了拟合,实验表明,随着pH的提高,平衡交换量增加,提高温度和SAM的初始浓度在一定程度上能促进内扩散过程,利用D113树脂在f 1.7×30.0cm小型柱上从酵母提取液中分离SAM的得率为90%以上,纯度达87%以上。The performance of ion exchange for S-Adenosyl-L-methionine (SAM) with weak acid resin D113 and its application on separating SAM from yeast lysates were investigated. The ion-exchange isotherm of SAM on D113 was correlated by Langmuir equation. The pH value influenced the ion-exchange amount greatly. Raising the temperature and the initial sample concentration were beneficial to improve the absorption rates. Process of separating SAM from yeast cell lysates on a mini column (f1.7×30.0 cm) filled with 50 ml D113 resin was evaluated and the SAM purity up to 87% with 90% recovery was reached.

关 键 词：S-腺苷-L-蛋氨酸 弱酸树脂 离子交换 酵母 分离 抗抑郁药 

分 类 号：TQ464.7[化学工程—制药化工] TQ028.33