非水毛细管电泳对9种手性药物的拆分  

Nonaqueous Capillary Electrophoresis for Chiral Separation of Nine Enantiomers

作　　者：初永宝[1] 蒋文强[2] 崔凤霞[2] 李关宾[2] 

机构地区：[1]山东大学环境科学与工程学院,山东济南250100 [2]山东轻工业学院食品工程系,山东济南250100

出　　处：《色谱》2003年第2期138-142,共5页Chinese Journal of Chromatography

基　　金：山东省自然科学基金资助项目(Q99B17).

摘　　要：用非水毛细管电泳成功地分离了洛贝林、托吡卡胺、普萘洛尔、阿替洛尔、环扁桃酯、沙丁胺醇、苯海索、芬氟拉明和异丙嗪等9种手性化合物。以甲酰胺为介质、柠檬酸 三羟甲基氨基甲烷(Tris)为电解质、在254nm波长下紫外检测。对手性选择剂的类型及浓度、背景电解质的pH值以及离子强度等因素对分离的影响做了系统的研究,最后确立了9种手性药物的最佳分离条件。实验发现,不同的手性选择剂对手性药物具有不同的分离选择性;每一种选择剂都存在一个相对水相体系较高的最佳浓度值而使分离效果最佳;在非水体系中,最佳pH值要比在水相体系中高;其分离效率也是随着体系中电泳介质离子强度的增大而升高。Chiral separation of nine enantiomers, which include lobeline, tropicamide, promethazine, propranolol, atenolol, cyclandelate, salbutamol, fenfluramine and benzhexol, has been for the first time achieved by nonaqueous capillary electrophoresis (NACE). Formamide was used as the background electrolyte solvent and citric acidTris as the electrolyte with the UV detection at 254 nm. In addition, the effects of the types of chiral resolving agents, the concentrations of the chiral resolving agent, apparent pH (pH*) and ionic strength on chiral separation in NACE were studied. Finally the optimal experimental conditions were established. The results demonstrate that different chiral resolving agents have different selectivities. For every enantiomer, the optimal concentration of chiral resolving agent was higher than that in aqueous medium. Such differences could arise from the fact that the electrostatic attraction caused stronger binding between the chiral resolving agent and the enantiomers. The optimal pH in the aqueous medium was lower than that in the solvent medium, which might be related to a shift in pKa of the solute in organic solvents. Higher electrical field strength and ionic strength could be applied in the nonaqueous solvent medium, and with the increase in ionic strength, the separation selectivity was further improved. 

关 键 词：手性药物 非水毛细管电泳 手性拆分 甲酰胺 分离 

分 类 号：R914[医药卫生—药物化学]