利用水相合成的量子点标记木瓜蛋白酶的研究  

Studies on Labeling of Papain with Quantum DotsSynthesized in Aqueous Solution

作　　者：林章碧[1] 张皓[2] 陈奇丹[1] 万异[1] 杨柏[2] 苏星光[1] 张家骅[3] 金钦汉[1] 

机构地区：[1]吉林大学化学学院分析科学研究所 [2]吉林大学超分子结构与材料教育部重点实验室,长春130023 [3]中国科学院激发态物理开放实验室,长春130021

出　　处：《高等学校化学学报》2003年第4期609-611,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 0 75 0 0 9)资助

摘　　要：The feasibility of the mercaptopropyl acid modified CdTe nanoparticles synthesized in aqueous solution to the labeling of biomoleculespapain via electrostatic interaction was studied in this paper. The absorption spectrum of CdTe papain solution became more flat than that of CdTe nanoparticles in the wavelength range from 400 to 600 nm. While the emission peak of CdTe papain solution underwent a red shift, the intrinsic emission band width was unchanged. The TEM images of monodisperse mercaptopropyl acid stabilized CdTe nanoparticles and CdTe papain solution were compared and the effect of pH of buffer solution was studied. It was proved that the red shift of the emission peak was the result of the electrostatic interaction between CdTe nanoparticles and papain.The feasibility of the mercaptopropyl acid modified CdTe nanoparticles synthesized in aqueous solution to the labeling of biomoleculespapain via electrostatic interaction was studied in this paper. The absorption spectrum of CdTe papain solution became more flat than that of CdTe nanoparticles in the wavelength range from 400 to 600 nm. While the emission peak of CdTe papain solution underwent a red shift, the intrinsic emission band width was unchanged. The TEM images of monodisperse mercaptopropyl acid stabilized CdTe nanoparticles and CdTe papain solution were compared and the effect of pH of buffer solution was studied. It was proved that the red shift of the emission peak was the result of the electrostatic interaction between CdTe nanoparticles and papain.

关 键 词：水相合成 半导体量子点 木瓜蛋白酶 静电相互作用 荧光检测 生物分子 生物医学 半导体纳米粒子 

分 类 号：Q556.9[生物学—生物化学]