对大鼠幼鼠背部膀胱经上缝隙连接分布状况的初步研究  

A Pillot Study of the Distribution of Gap Junctions along the Dorsal Urinary Meridian in Young Rats

作　　者：袁冬 朱蓬第 祝总骧[2] 徐瑞民[2] 

机构地区：[1]国家计生委科研所,北京100081 [2]北京经络研究中心,中国科学院生物物理所北京100006

出　　处：《中国中医基础医学杂志》2003年第1期50-52,54,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家科技部"八五"攀登项目

摘　　要：目的 :从微观上检测大鼠幼鼠背部膀胱经表皮细胞层的缝隙连接在经脉线上与线外差别 ,为阐明作用机理和物质基础提供依据。方法 :采用硝酸镧示踪法 ,在超微结构水平 ,对 10只大鼠幼鼠背部膀胱经上和经外对照区表皮细胞层缝隙连接做了比较性研究 ,并用生物体视学中的方网格和平行线两种测试系统对其进行了体视学测量。结果 :发现经脉线上和经脉线外对照区表皮细胞层缝隙连接的二维、三维 8个参数均差异明显 (P <0 0 0 1,F检验 )。结论 :大鼠幼鼠背部膀胱经上表皮细胞层缝隙连接相对集中是新发现的又一项经脉线下的形态学特征。Objective:To investigate the distribution of gap junction in the epidermal cell layer along and outside(control) the dorsal Urinary Bladder meridian in young rats.Methods:Using the Lanthanum-nitrate labeled on the ultrastructural level,a comparative study of gap junctions in the epidermal cell layer along and outside(control) the dorsal Urinary Bladder Meridian has been investigated in 10 young rats.In addition,a stereometry was carried out by means of the two Bio-stereological assessment systems:the square-net-check and the parallel-line methods.Results:There were significant differences in the 8 parameters(two dimensions and three dimensions)in the epidermal cell layer on the gap junctions between the meridian and the control.Site( P<0 001,F test).Conclusion:The more concentrated gap junctions of the epidermal cells along the Urinary Bladder Meridian in young rats was another morphological characteristic which was first discovered in this study.

关 键 词：经脉线 缝隙连接 低阻抗 生物体视学 表皮层 大鼠幼鼠 

分 类 号：R224.1[医药卫生—中医基础理论]