转染外源性p16基因对大鼠恶性胶质瘤的生长抑制作用  

In vivo inhibition of murine malignant glioma cells by exogenously transfected p16

作　　者：焦保华[1] 浦佩玉[2] 吴立华[1] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄050000 [2]天津医科大学总医院神经外科,天津300052

出　　处：《基础医学与临床》2003年第1期63-67,共5页Basic and Clinical Medicine

基　　金：河北省科委科研基金 (0 0 2 76 170D)

摘　　要：探讨p16基因在体内对恶性胶质瘤的生长抑制作用。将外源性p16基因导入C6细胞内 ,应用立体定向技术将C6细胞种植于SD大鼠颅内尾状核头部 ,用核磁共振 (MR)扫描技术 ,动态观察颅内肿瘤生长情况。并通过免疫组化、原位杂交和细胞凋亡检测肿瘤细胞的增殖活性。结果显示 ,转染组和治疗组大鼠生存期较对照组明显延长。治疗组肿瘤随时间的延长逐渐缩小。免疫组化显示转染组和治疗组p16蛋白表达明显增强。原位杂交和细胞凋亡检测表明 ,转染组和治疗组大鼠肿瘤细胞增殖活性降低。本实验表明 ,p16基因在体内有抑制恶性胶质瘤生长的作用 ;瘤体内注入p16cDNA质粒 脂质体复合物 ,可使肿瘤生长受到明显抑制 。To study the effect of exogenous gene p16 on the growth of murine malignant glioma cells in vivo . Exogenous p16 was transfected into rat malignant glioma cells (C 6) which were transplanted into the head of caudate nucleus of SD rats with stereotactic technology. The growth of brain tumor was observed with magnetic resonance image (MRI). The proliferative activity of tumor cells was examined by immunohistochemical method, hybridization in situ and apoptosis test. The survival time of the transfection group and treatment group in rats was significantly longer than that of the controls. With the running of time, the tumor size of treatment group gradually reduced.Immunohistochemistry showed an enhanced expression of p16 protein both in transfection and controls. Hybridization in situ and apoptosis assay indicated that proliferative activity of tumor cells decreased in the transfection and treatment groups. The present results demonstrated that p16 gene had the same effect to inhibit growth of malignant glioma in vivo . When p16 cDNA was injected into tumors,it inhibited these tumors from development and reject the tumor.

关 键 词：外源性P16基因 脑胶质瘤 体内治疗 

分 类 号：R739.4[医药卫生—肿瘤]