阳离子脂质体包裹CpG对猪瘟猪肺疫猪丹毒三联疫苗免疫效应的影响  

Immune effect analysis of the mice mediated by cationic liposome entrapped CpG motif and the tri-vaccine of swine fever, lung pestilence and porcine erysipelas

作　　者：龙章富[1] 高荣[1] 孟民杰[1] 武梅[1] 李江凌[1] 沈翼[1] 巫雪艳[1] 王丽焕[1] 谢鸿观[1] 殷雪[1] 刘昆[1] 郑勇[1] 刘世贵[1] 

机构地区：[1]四川大学草原生物防治工程国家专业实验室,四川成都610064

出　　处：《中国兽医科技》2003年第3期15-19,共5页Chinese Journal of Veterinary Science and Technology

基　　金：教育部重点项目 (0 0 1 0 4 );四川省"十五"重大科技攻关项目 (0 1NG0 1 8 0 1 )

摘　　要：对 3组分别注射猪瘟猪肺疫猪丹毒三联疫苗 (简称三联疫苗 )、CpG +三联疫苗、阳离子脂质体包裹CpG +三联疫苗的小鼠细胞和体液免疫反应进行了比较分析。结果显示 ,阳离子脂质体包裹CpG +三联疫苗免疫小鼠脾细胞增殖反应明显增强 ,白细胞介素 2 (IL 2 )诱生活性和免疫细胞数量、血液IgG含量较仅注射三联疫苗的小鼠显著增加。说明阳离子脂质体包裹CpG能增强小鼠对三联疫苗的体液和细胞免疫应答反应 。The results showed that the CpG motif coated with cationic liposome can enhance the cellular and humour immune response abilities to the protein vaccine which named swine fever, lung pestilence and porcine erysipelas, a type of tri vaccine. The groups of mice injected with CpG combined with tri vaccine or cationic liposome entrapped CpG plus tri vaccine is higher in spleen cell proliferation abilities, the induced activities of IL 2 and immune cell numbers than only treated with the tri vaccine (control group). This suggested that cationicliposome entrapped CpG motif is a better immune adjuvant of the swine tri vaccine, a protein molecular vaccine.

关 键 词：阳离子脂质体 CPG 猪瘟 猪肺疫 猪丹毒 三联疫苗 免疫效应 

分 类 号：S858.28[农业科学—临床兽医学]