复合应激时兔血浆与胃十二指肠黏膜中胃动素含量变化及其机制  

Effects of combined stress on rabbit serum and gastroduodenal mucosa motilin and its mechanism

作　　者：陈光忠[1] 罗炳德[1] 翟惠敏[2] 王红芹 邹飞[1] 

机构地区：[1]第一军医大学高温医学研究室,广东广州510515 [2]第一军医大学南方医院护理部,广东广州510515 [3]山东省莱阳市第二人民医院心内科,山东莱阳265200

出　　处：《中国危重病急救医学》2003年第4期229-231,共3页Chinese Critical Care Medicine

基　　金：军队"十五"指令性课题 (0 1L 0 5 1)

摘　　要：目的 :研究兔血浆及胃十二指肠黏膜中胃动素 (MTL)的分布情况 ,探讨高温和创伤复合应激对MTL 含量的影响及其机制。方法 :将实验动物随机分为正常对照组、常温 (2 3℃ )创伤组、高温 (38℃ )无创组和高温有创组。用放射免疫法测定正常兔血浆及胃十二指肠不同部位黏膜中 MTL 的含量 ;建立高温创伤动物模型 ,于 0 h、0 .5 h、1h、1.5 h及 2 h采血测定血浆中 MTL的含量 ,并观察其动态变化。结果 :正常对照组胃体黏膜中 MTL含量最高 ,其次为胃底、幽门、十二指肠 ;血浆中 MTL含量高于组织中含量。高温及创伤应激后 ,组织中 MTL含量降低 ,血浆中 MTL含量升高。结论 :胃十二指肠不同部位黏膜中 MTL含量不同 ,以胃体黏膜中含量最高 ;高温创伤应激可影响 MTL 在组织和血浆中的分布 ,且呈反向变化。其可能机制为 Mo细胞受到破坏导致 MTL 释放以及应激状态下体内蛙皮素增多促使 MTL 分泌增加等因素所致。Objective:To study the effects of heat exposure and trauma stress on the level of motilin (MTL) in plasm and the distribution of MTL gastroduodenal mucosa and its mechanism in rabbits.Methods:The rabbits were randomly divided into four groups,control group,trauma group(23 ℃),heat exposure group(38 ℃) and heat exposure combined trauma group.The MTL was measured by radioimmunoassay(RIA).The rabbit model of heat exposure with trauma was established.The levels of the MTL were measured at 0,0.5,1,1.5 and 2 hours during experiment.Results:In control group,the concentration of MTL was hightest in gastric corpus,and then were gastric fundus,pylorus and Duodenal mucous membrane.The concentration of MTL was higher in plasm than that in tissues.After heat exposure and trauma,the concentration of MTL decreased in tissues and increased in plasm.Conclusion:The concentration of MTL was different in different parts of gastroduodenal mucosa.The heat exposure and trauma stress may result in the converse changes of the level of MTL in plasma and tissues.The mechanism might be that the destroyed Mo cells released MTL and increasing level of bombesin stimulated by stress induced the release of MTL.

关 键 词：消化道黏膜 胃动素 热应激 创伤应激 

分 类 号：R363[医药卫生—病理学]