极端嗜热古菌—芝田硫化叶菌反向旋转酶的快速纯化  

Purification of a Reverse Gyrase from the Hyperthermophilic Archaeon Sulfolobus Shibatae Using a Simple Procedure

作　　者：戴鹏高[1] 黄力[1] 

机构地区：[1]中国科学院微生物研究所微生物资源前期开发国家重点实验室,北京100080

出　　处：《微生物学报》2003年第2期241-244,共4页Acta Microbiologica Sinica

基　　金：国家自然科学基金重点项目 (3 0 0 3 0 0 1 0 );杰出青年基金 (3 992 5 0 0 1 )资助

摘　　要：反向旋转酶是一种I型拓扑异构酶 ,它可以利用ATP水解的能量向DNA分子中引入正超螺旋。通过阴离子交换层析、亲和层析、聚丙烯酰胺凝胶电泳 (SDS PAGE)从芝田硫化叶菌 (Sulfolobusshibatae)中分离得到一种反向旋转酶。SDS PAGE显示 ,该酶分子量约为 1 2 6kD ,N 末端序列测定结果表明 。Reverse gyrases are type I topoisomerses capable of introducing positive supercoils into DNA at the expense of ATP. By using ion exchange, heparin agarose chromatography and SDS PAGE, a reverse gyrase was purified to homogeneity from the hyperthermophilic achaeon \%Sulfolobus shibatae \% The molecular mass of the enzyme is ～126 kD, as judged by SDS PAGE. N terminal amino acid sequence analysis shows that the enzyme is different from a reverse gyrase isolated previously from \%S. shibatae. \%

关 键 词：极端嗜热古菌 拓扑异构酶 反向旋转酶 SDS-PAGE 纯化 

分 类 号：Q936[生物学—微生物学]