同种异体宫内移植小鼠嵌合模型的建立(英文)  

A Chimeric Mouse Model Established by Allogenic in utero Transplantation

作　　者：王墨林[1] 颜景斌[1] 肖艳萍[1] 任兆瑞[1] 黄淑帧[1] 曾溢滔[1] 

机构地区：[1]上海交通大学附属儿童医院上海医学遗传研究所,上海200040

出　　处：《Acta Genetica Sinica》2003年第4期289-294,共6页

基　　金：国家"8 63"计划资助项目 (批准号 :2 0 0 2AA2 160 91)~~

摘　　要：干细胞宫内移植是一种很有前途的产前治疗方式。为深入研究干细胞移植后的细胞行为 ,采用宫内移植的方法建立同种异体的嵌合小鼠模型。将雄鼠骨髓单核细胞宫内注射到胎鼠腹腔 ,在受体鼠出生后检测雌性受体鼠外周血细胞。应用PCR检测外周血是否存在雄性鼠的DNA ,并采用定量PCR技术确定其嵌合量 ;同时用荧光原位杂交 (FISH)技术直观观察外周血中雄性来源的细胞。结果表明 :共获得 4只阳性外周血嵌合小鼠 ,其中 3只稳定嵌合达到 6个月以上。应用宫内移植成功建立了外周血中存在异源细胞的小鼠嵌合模型。In utero stem cells transplantation is a promising approach to the prenatal treatment of diseases.In order to investigate the fate of the stem cells after in utero transplantation,we have established a chimeric mouse model with the method of in utero transplantation.Mononuclear cells (including stem cells /progenitor cells) derived from male mouse bone marrow were injected into fetal mouse peritoneal cavity during the pre immune period.The donor cells in the circulatory blood of female recipients were identified by fluorescence in situ hybridization (FISH),and the Y chromosome specific DNA was detected by PCR as well as real time quantitative PCR after the recipient mice were born.The results showed that a total of 4 female recipient mice were chimeric in their peripheral blood.Significantly,the donor cells in three chimeric mice persisted up to six months.

关 键 词：干细胞 移植 宫内 小鼠 嵌合体 

分 类 号：R-332[医药卫生]