应激状态下大鼠胃黏膜适应性细胞保护作用的研究  

Relation between nitric oxide synthase,psychology,gastric mucosal blood flow and gastric mucosal adaptive cytoprotection under stress in rats

作　　者：聂时南[1] 钱晓明[1] 唐文杰[1] 李兆申[2] 

机构地区：[1]南京军区总医院 [2]第二军医大学长海医院消化内科,上海200433

出　　处：《中国危重病急救医学》2003年第4期222-225,共4页Chinese Critical Care Medicine

基　　金：全军"十五"医药科研基金重点项目 (0 1Z0 5 9)

摘　　要：目的 :探讨心理、一氧化氮合酶 (NOS)及胃黏膜血流量 (GMBF)与应激状态下大鼠胃黏膜适应性细胞保护相互作用及意义。方法 :采用高压恒流脉冲刺激器复制胃黏膜损伤模型 ,将 72只雄性 SD大鼠随机分为对照组、规则光组及不规则光组 3组 ,分光光度法检测 NOS活性 ,动态检测 GMBF及 Nils L ambecht法判定胃黏膜损伤指数 (II)。结果 :在两个应激组中 ,NOS均升高 ,GMBF均下降后回升 ,而黏膜损伤均渐趋减轻。在规则组中 ,II与 GMBF呈显著负相关 ,在不规则组中 ,II与 NOS和 GMBF均呈显著负相关 ,而 NOS与 GMBF呈显著正相关。结论 :NOS、GMBF及心理共同参与了胃黏膜适应性细胞保护反应。Objective:To explore the relation between nitric oxide synthase(NOS),psychology,gastric mucosal blood flow(GMBF) and gastric mucosal adaptive cytoprotection under stress in rats and its possible mechanism.Methods:Animal model was constructed with impulsive stimulator of high voltage and stable currency, seventytwo male SD rats were randomly divided into three groups:control(C),regular(R) and irregular(I) group.NOS activity was measured by spectrophotometry and injure of gastric mucosa was measured by method of Nils Lambecht,GMBF was measured by LDF3 flowmeter. Results:In both R group and I group,NOS increased significantly,but injury index(II) was on contrary apparently decreasing.GMBF was increased gradually after stress.In R group,there was a negative proportion between II and GMBF,in I group,there was a negative proportion between II and GMBF and NOS,but there was a positive relationship between NOS and GMBF.Conclusion:NOS,psychology and GMBF all might participate in the course of the gastric mucosal adaptive cytoprotection under stress.

关 键 词：一氧化氮合酶 胃黏膜损伤 躯体性应激 心理性应激 胃黏膜血流量 适应性细胞保护 

分 类 号：R363[医药卫生—病理学]