P_(53)和bcl-2在老年性白内障晶体上皮细胞中蛋白表达的研究  

The protein expression of P_(53) and bcl-2 in lens epithelial cells of senile cataract

作　　者：李元彬[1] 陈薇[2] 朱风云[1] 李守明[1] 杨宪勇[1] 

机构地区：[1]泰安市中心医院眼科,271000 [2]山东大学医学院

出　　处：《中国实用眼科杂志》2003年第3期180-183,共4页Chinese Journal of Practical Ophthalmology

摘　　要：目的 :研究P53 ,Bcl -2基因在老年性白内障晶体上皮细胞中的蛋白表达 ,探讨P53 ,bcl -2在老年性白内障晶体上皮细胞凋亡中的作用。方法 :应用免疫组织化学方法 ,对 3 0例老年性白内障及 10例正常透明晶体上皮细胞进行P53 、bcl-2基因蛋白表达的检测 ,计算P53 、bcl-2基因蛋白表达阳性细胞百分率。结果 :P53 在正常晶体上皮细胞中无蛋白表达 ,在老年性白内障晶体上皮细胞中蛋白表达率为 16 9～ 19 1% ,bcl -2在两组中均无蛋白表达。结论 :P53 在老年性白内障晶体上皮细胞中蛋白高表达 ,P53 可能促进了老年性白内障中晶体上皮细胞的调亡。Objective:To study the protein expression of P53 and Bcl-2 in the lens epithelial cells of senile cataract,and to assess the relationships between P53,Bcl-2 and the apoptosis of senile cataract lens cells. Methods:With the SABC methods,staining by using DAB,detective the protein expression positive cell rate of P53 and Bcl-2. Results:In the epithelial cells of the normal lens,there was almost no P53 protein expression,but in the cells of the senile cataract lens,the rate of positive cells of P53 protein expression was 16 9～19 1%.For Bcl-2 protein expression,no positive cells were seen in both the two kinds of epithelial cells. Conclusions:P53 protein expression is higher in senile cataract lens epithelial cells.P53 may promote the apoptosis in the lens epithelial cells of senile cataract.

关 键 词：P53 BCL-2 老年性白内障 晶体上皮细胞 蛋白表达 研究 

分 类 号：R776.1[医药卫生—眼科]