毫米波照射对人肝癌细胞株BEL 7404凋亡的影响  

Effect of millimeter wave radiation on apoptosis of human hepatoma cells

作　　者：戎兰[1] 孙大裕[1] 梅玲[1] 陆玮[1] 

机构地区：[1]复旦大学附属华山医院消化科,上海200040

出　　处：《上海医学》2003年第2期130-132,I003,共4页Shanghai Medical Journal

摘　　要：目的　研究 35 .8GHz毫米波照射能否诱导人肝癌细胞株BEL 74 0 4凋亡及其可能的机制。方法　选取BEL 74 0 4人肝癌细胞体外培养 ,随机分为 4组 ,采用荧光显微镜和电镜观察细胞核的变化 ;采用免疫印迹法检测半胱氨酸蛋白酶 (caspase) 3的底物多聚腺苷酸核糖基化聚合酶 (PARP)被剪切的比例。结果　BEL74 0 4细胞经毫米波照射后诱发的细胞死亡具有细胞凋亡的形态学特征 ,其中毫米波和 5 氟脲嘧啶 (5 Fu)联合组细胞凋亡比例增高 ;免疫印迹分析对照组中相对分子质量为 116× 10 3 的PARP几乎完全保持完整 ,经毫米波或 5 FU处理过的BEL74 0 4细胞中 ,116× 10 3 的PARP被切割成相对分子质量为 85× 10 3 的肽段 ,其中以毫米波和 5 Fu联合组被剪切的PARP比例最高。结论　毫米波照射可诱导BEL74 0 4肝癌细胞凋亡 ,与 5Objective To study the effect of millimeter wave radiation on human hepatoma cells.Methods BEL7404 hepatoma cells were cultured in vitro and randomly divided into 4 groups. The ability of 35.8 GHz millimeter wave to induce the apoptosis of hepatoma cell was confirmed by the analyses of fluorescence and electron microscopy. The mechanism of cell apoptosis was investigated by immuno blotting analysis.Results The BEL7404 cells possessed the typical characteristics of apoptosis by the millimeter wave radiation under fluorescence and electron microscopy. PARP was found to be cleavaged in all the cells in millmeter wave radiation or/and 5 Fu groups.Conclusion Radiation of 35.8 GHz could induce apoptosis of BEL7404 cell in vitro, and in combination with 5 Fu would be more efficient.

关 键 词：毫米波 照射 肝癌 细胞株 BEL7404 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]