丙丁酚对晚期糖化终产物刺激的微血管内皮细胞血管细胞粘附分子-1表达的影响  

Probucol inhibits AGEs-induced VCAM-1 expression on mouse cardiac microvascular endothelial cells

作　　者：徐茂锦[1] 邹大进[1] 叶江洪[2] 

机构地区：[1]第二军医大学长海医院内分泌科,上海200433 [2]解放军411医院内一科

出　　处：《上海医学》2003年第2期136-137,共2页Shanghai Medical Journal

摘　　要：目的　研究丙丁酚对晚期糖化终产物 (AGEs)诱导的微血管内皮细胞血管细胞粘附分子 1(VCAM 1)表达的影响及作用机制。方法　采用流式细胞仪测定AGEs刺激及丙丁酚作用后小鼠心脏微血管内皮细胞VCAM 1的表达 ,采用放射免疫法测定培养上清液中肿瘤坏死因子 (TNF) α水平。结果　AGEs刺激后VCAM 1表达增加 ,TNF α水平升高 ;丙丁酚干预组VCAM 1表达降低 ,TNF α水平下降并具有剂量依赖性。结论　丙丁酚能抑制AGEs诱导的微血管内皮细胞VCAM 1的表达 ,TNF α的介导是可能的机制之一。Objective To study the effect of probucol on AGEs induced VCAM 1 expression on mouse cardiac microvascular endothelial cells and its possible mechanism. Methods The VCAM 1 expression of endothelial cells was measured through FACS after induction by AGEs and given probucol, the TNF α level of cultiured supernatant was measured by RIA. Results The VCAM 1 expression and TNF α level increased significantly after induction by AGEs induced and decreased in a dose dependent manner after given probucol. Conclusion Probucol inhibits AGEs induced VCAM 1 expression on mouse cardiac microvascular endothelial cells and TNF α might be the mechanism involved.

关 键 词：丙丁酚 晚期糖化终产物 刺激 微血管 内皮细胞 血管细胞粘附分子-1 肿瘤坏死因子 糖尿病 

分 类 号：R363[医药卫生—病理学]