清热活血健脾中药对大鼠肝癌基因转录差异的调整  

Regulation of gene transcription in rat with liver cancer by traditional Chinese medicine

作　　者：方肇勤[1] 管冬元[1] 梁尚华[1] 

机构地区：[1]上海中医药大学基础医学院,上海市200032

出　　处：《世界华人消化杂志》2003年第3期276-280,共5页World Chinese Journal of Digestology

基　　金：国家中医药管理局科研基金;NO.97Z014~~

摘　　要：目的:观察中药抑癌扶正行气活血方(全方)及其不同治法的拆方:清热方、活血方、健脾方对肝癌大鼠的作用和对肝组织基因转录的整体调节作用.方法:采用二乙基亚硝胺(DEN)诱发大鼠肝癌,设正常组、模型组、不同中药组及FT-207组对照,分别观察各组大鼠生存率、肝、体、肝/体及肝组织的病理、甲胎蛋白(AFP)免疫组化变化;以及采用DDPCR技术显示正常肝组织与模型组肝癌组织中呈差异转录的cDNA片段,Northernblot验证这些cDNA片段在各组中转录的差异,并将呈差异转录的cDNA片段进行克隆与测序.结果:全方其及拆方均能不同程度地改善肝癌大鼠的一般情况,提高大鼠的生存率.各拆方中以清热组与活血组为优;全方其及拆方均能不同程度地抑制肿瘤的生长及肝脏AFP的合成,其中以全方组与清热组为优;采用DDPCR结合Northernblot筛选出在各组中呈显著差异转录的9个阳性cDNA片段,经与Genbank比较4个为新的基因,且这9个基因在肝癌发生与转归中的作用至今未见报道;全方及其拆方对这些基因的转录水平有不同程度的调节作用,其中DD29下调57-78%,DD11、DD25也有下调至60%和78%,使基因转录水平接近正常肝组织.结论:全方及其不同治法的拆方中药对大鼠肝癌具有直接治疗作用,能不同程度地广泛地调控肝组织(含肝癌组织)有关基因的转录水平.AIM:To observe the regulatory effects of YAF Compositus and its modified Compositus: Gingrefang, Huoxuefang and Jianpifang on liver cancer and their transcription the genes in rats. METHODS:DEN was used to induce the liver cancer in rats. All rats were divided into seven groups:normal group, model group, four therapy groups and FT-207 group. The survival rate, liver weight, body weight, liver weight/body weight, and pathological change of liver tissues were observed. Immunocytochemical change of AFP and DD-PCR was used to display the different transcriptional expression of cDNA fragments between normal liver tissues and DEN-induced liver cancer tissues. Northern blotting was used to verify the different transcription of these cDNA fragments. RESULTS:Both YAF Composita and its modified Composita could improve the general condition of rats with liver cancer, especially Qingrefang and Huoxuefang. Different Composita inhibited the growth of cancer and synthesis of AFP, especially YAF Composita and Qingrefang. 9 genes important for the transformation of cancer were selected with DD-PCR and Northern blotting. YAF Composita and its modified Composita had capabilities to regulate differently the genetic transcription: DD-29 decreased 51-78 %, DD11 and DD-25 decreased 60 % and 78 % respectively, near to that of normal liver tissue. CONCLUSION:Both YAF Composita and its modified Composita can be directly used to treat the liver cancer, and to regulate the transcription of the different genes in the tissues.

关 键 词：清热活血健脾中药 大鼠 肝癌 基因转录差异 调整 

分 类 号：R735.7[医药卫生—肿瘤]