蜂胶对小鼠移植瘤细胞株生长的抑制作用的形态学观察  

Morphologic Study of Inhibitory Effects of Propolis on Cell Growth of Transplan tation Tumor in Mice

作　　者：郭文君[1] 韩连堂[1] 王春梅[2] 王志萍[1] 魏志新[1] 高建华[1] 邱玉刚[1] 李新伟[1] 

机构地区：[1]潍坊医学院,潍坊市261042 [2]潍坊市中医院,潍坊市261042

出　　处：《中国药房》2003年第4期206-207,共2页China Pharmacy

摘　　要：目的 :探讨蜂胶对小鼠移植瘤 (S180)生长的抑制作用。方法 :用不同浓度的蜂胶饲料分组喂养小鼠2月后 ,在其腋下接种肿瘤细胞株 (S180) ,8天后 ,手术取出肿瘤组织称重 ,石蜡切片 ,HE染色 ,显微镜下观察形态学变化并在高倍镜下计数肿瘤细胞核分裂像。结果 :蜂胶实验组小鼠移植瘤重量较对照组显著减轻 (P<0 01) ,肿瘤生长抑制率B组为45 7% ,C组为66 0% ,D组为64 4 %。实验组肿瘤组织内核分裂像较对照组显著减少 (P<0 01) ,肿瘤组织周围淋巴细胞呈反应性增生。结论 :适量蜂胶饲养小鼠 ,对其移植瘤细胞株的生长有抑制作用。OBJECTIVE:To study the inhibitory effects of propolis on growth of transplantation tumor in mice.METHO_ DS:Using different concentrations of propolis to feed the mice for two months,the tumor cells(S 180 )were transplanted into subaxillary tissue of the mice.After8days,the tumor mass was takent off the body of the mice,and weighted,then paraffin sections were observed and the number of karyokinesis of tumor cells was counted under the microscops.RESULTS:The weight of tumor mass were lighter in the propolis group than in the control group(P<0.01).The inhibitory rates of tumor growth in group B,C and D were45.7%,66.0%and64.4%respectively.The number of karyokinesis of tumor cells was less in the propolis group than in the control group(P<0.01).The lymphocyte surrounding tumor tissure assumed reactive proliferaˉtion.CONCLUSION:The propolis might effectively inhibit the growth of transplantation tumor in mice.

关 键 词：蜂胶 小鼠 移植瘤细胞株 抑制作用 形态学观察 

分 类 号：R73－36[医药卫生—肿瘤]