Study on Frequency Doubling Scattering and Second-Order Scattering Spectra of Heparin-Methylene Blue System  

作　　者：刘绍璞 罗红群 李念兵 刘忠芳 

机构地区：[1]Institute of Environmental Chemistry, Southwest China Normal University

出　　处：《Chinese Journal of Chemistry》2003年第4期423-428,共6页中国化学（英文版）

基　　金：theNationalNaturalScienceFoundationofChina(No .2 98750 19);theMunicipalScienceFoundationofChongqingCity(No.20027472)

摘　　要：Binding of heparin with methylene blue (MB) in pH 5.7 Britton Robinson buffer can result in a significant enhancement of frequency doubling scattering (FDS) and second order scattering (SOS). Their maximum scattering wavelengths ( λ max ) appear at 350 nm for FDS and 700 nm for SOS, respectively. The optimum conditions of the reaction, the influencing factors and the relationship between the two scattering intensities and the concentration of heparin have been investigated. The new methods for the determination of trace amounts of heparin based on the FDS and SOS methods have been developed, which exhibit high sensitivities. The detection limits of heparin are 4.36 ng/mL for the FDS method and 3.55 ng/mL for the SOS method, respectively. Both of the methods have fairly good selectivity and were applied to the determination of heparin in sodium heparinate injection samples with satisfactory results. Moreover, the relative mechanisms have also been discussed.Binding of heparin with methylene blue (MB) in pH 5.7 Britton Robinson buffer can result in a significant enhancement of frequency doubling scattering (FDS) and second order scattering (SOS). Their maximum scattering wavelengths ( λ max ) appear at 350 nm for FDS and 700 nm for SOS, respectively. The optimum conditions of the reaction, the influencing factors and the relationship between the two scattering intensities and the concentration of heparin have been investigated. The new methods for the determination of trace amounts of heparin based on the FDS and SOS methods have been developed, which exhibit high sensitivities. The detection limits of heparin are 4.36 ng/mL for the FDS method and 3.55 ng/mL for the SOS method, respectively. Both of the methods have fairly good selectivity and were applied to the determination of heparin in sodium heparinate injection samples with satisfactory results. Moreover, the relative mechanisms have also been discussed.

关 键 词：frequency doubling scattering second  order scattering HEPARIN methylene blue 

分 类 号：O657.3[理学—分析化学]