抗人血管内皮生长因子单克隆抗体抑制肉瘤生长的实验研究  

Inhibition of Experimental Mouse Fibrosarcoma Growth by a Monoclonal Antibody to Human Vascular Endothelial Growth Factor

作　　者：王大章[1] 房思炼[2] 廖楚航[1] 郑光勇[1] 刘曙光[1] 李鹏[1] 

机构地区：[1]四川大学华西口腔医学院,口腔颌面外科,生物医学工程重点实验室 [2]中山大学附属二院博士后流动站,广东广州510020

出　　处：《中国口腔颌面外科杂志》2003年第1期35-39,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(39970798)

摘　　要：目的观察抗人VEGF单抗E11对小鼠肉瘤S180生长的影响。方法BALB/c小鼠45只,接种小鼠肉瘤细胞株S180,随机均分为5组。其中3组于接种后1～4、7～11天,分别通过腹腔或瘤周皮下注射抗人VEGF单抗E11100μg、200μg。另以生理盐水及5-Fu注射作阴性及阳性对照组。接种后第14天处死小鼠并称取瘤重,计算抑瘤率,并做组织病理学检查。结果抗人VEGF单抗皮下及腹腔给药均可抑制S180肉瘤的生长,且抗人VEGF单抗200μg/d组抑瘤率均高于5-Fu组;皮下给药组抑瘤率最高,达67.2%。结论不同剂量、不同给药途径的抗人VEGF单抗对小鼠肉瘤S180的生长均有明显抑制作用,说明抗人VEGF单抗可通过阻断VEGF的作用,抑制肿瘤血管生成,从而达到抑制肿瘤生长的目的。提示抗人VEGF单抗具有可能的临床应用前景。Objective The purpose of this study was to apply the anti-VEGF MAb(E11),which was generated against a synthetic peptide from the NH 2 of the human VEGF(residues1-26),to against VEGF in the mice fibrosarcoma S180and observe the inhibition effect of tumor growth.Methods The mice fibrosarcoma cell lines S180was injected s.c.into BALB/c mice,and then these mice were randomized into three groups.The anti-human VEGF MAb was administered i.p.or s.c.with100μg or200μg each injection on days1-4,7-11.Normal saline or5-Fu was administrated i.p.separately as control groups.Mice were sacrificed on14th day.The tumor weight was determined and the inhibition rate was calculated.Results The growth of S180fibrosacoma in MAb-treated group was dramatically inhibited,especially in MAb adminis-trated s.c.group.The tumor growth inhibition rate in mice treated s.c.with MAb reached67.2%.Conclusion The anti-human VEGF MAb had a significant inhibitory effect on growth of mouse fibrosarcoma by blocking the action of VEGF,without marked side effects.The results indicated that VEGF played an important role as a tumor angiogenesis factor,and the anti-human VEGF MAb had a potential application for clinical treatment of malignant tumors.

关 键 词：血管内皮生长因子 血管生成 单克隆抗体 肿瘤生长 VEGF 组织病理学 

分 类 号：R73-36[医药卫生—肿瘤]