钙离子对沙土鼠脑缺血再灌流后海马区胶质纤维酸性蛋白的作用  

Effect of Ca^(2+) on GFAP Production in the Gerbil Hippocampus After Recirculation Following Ischemia

作　　者：张敬军[1] 夏作理[1] 刘焯霖[2] 

机构地区：[1]泰山医学院附属医院神经内科,山东省泰安市271000 [2]中山大学附属第一医院神经内科,广东广州510080

出　　处：《中国微循环》2002年第5期280-282,共3页Journal of Chinese Microcirculation

基　　金：山东省自然科学基金 (NOY2000C23)资助课题 ;山东省省级中青年学术骨干基金 (369)资助

摘　　要：目的探讨钙离子对沙土鼠脑缺血再灌流后海马区胶质纤维酸性蛋白(GFAP)合成的影响。方法钳夹沙土鼠的双侧颈总动脉制造脑缺血模型 ,体外孵育海马组织脑片 ,应用免疫荧光法染色 ,观察GFAP浓度的变化及分布。结果脑缺血再灌流后钙离子增加海马区GFAP的合成 ,GFAP阳性细胞主要分布在放射层及分子层。Objective To observe effects of Ca2+ on glial fibrillary acidic protein (GFAP) production in the gerbil hippocampus after recirculation following ischemia.Methods Both vessels occlusion and immunofluorescent methods in gerbil hippocampal tissue slices incubated in vitro were used to observe change of GFAP concentration and distribution. Results Ca2+ leads to a rise on GFAP concentration in the hippocampus after recirculation following ischemia.GFAP positive cells mainly distributed in rediatum layer and molecullar layer in the hippocampus. Conclusions There is relation between Ca2+ and GFAP production.

关 键 词：钙离子 沙土鼠 脑缺血再灌流 海马区 胶质纤维酸性蛋白 CNP 

分 类 号：R743.3[医药卫生—神经病学与精神病学]