邻硝基苯基荧光酮与锌显色反应的研究  

Spectrophotometric Determination of Trace Amounts of Zn(II) with 0-NPF

作　　者：杨奉真[1] 董云会[1] 衣光舜[1] 

机构地区：[1]山东理工大学,山东淄博255091

出　　处：《淄博学院学报（自然科学与工程版）》2002年第3期57-59,共3页Journal of Zibo University(Natural Sciences and Engineering)

摘　　要：在pH=8.51的硼砂——硼酸缓冲体系、CPB为表面活性剂的条件下,Zn（Ⅱ）与邻硝基苯基荧光酮（0-NPF）形成1:4的紫红色络合物,最大吸收波长为550nm,灵敏度（ε）为1.25×105L.mol-1.cm-1.络合物在常温下能稳24小时以上,含锌量在0～20μg/50ml范围内符合比耳定律.在掩蔽剂存在下,有较好的选择性,测定大米、人发中锌的含量,结果令人满意.A spectrophotometric method for the determination of Zn(II) with 0-NPF is described in pH = 8.51 borax-boric acid buffer medium. Zn(II) reacts with 0-NPF to form a 1 : 4 complex in the presence of surfactant CPB. The molar absorbance measured to be 1. 25 × 105L. mol-1. cm-1 at 550nm. Beer law is obeyed in the range of 0-20μg/50ml. Trace amounts of Zn(II) in rice and hair have been determined by the proposed method and the results obtained are satisfactory.

关 键 词：邻硝基苯基荧光酮 显色反应 痕量锌 测定 大米 人发 

分 类 号：Q581[生物学—生物化学] Q503