葡萄膜炎患者外周血单核细胞表达的疾病相关细胞因子的检测  

Determination of the Disease Correlative Cytokines Expressed by Peripheral Blood Mononuclear Cells from Patients of Uveitis

作　　者：乔治[1] 闵磊[1] 张梁海 

机构地区：[1]武汉大学医学院,湖北武汉430071 [2]武汉医学研究所,湖北武汉430014

出　　处：《武汉大学学报（理学版）》2002年第6期729-731,共3页Journal of Wuhan University:Natural Science Edition

摘　　要：用ELISA测IL 12、IFN γ水平 ;用原位杂交染色法测IL 12mRNA表达 ;电泳迁移率改变试验 (EM SA)测NF AT活性 结果表明 :葡萄膜炎患者PBMCIL 12、IFN γ水平和IL 12mRNA表达以及NF AT活性都显著高于正常对照和其他眼疾病患者 (P <0 .0 1) IL 12、IFN γ水平异常增高是葡萄膜炎患者Th1/Th2 功能紊乱、Th1细胞功能亢进主要原因之一 ;而在转录水平上NF AT活性异常增高可能与患者IFN γ基因转录增强密切相关.To detect the The levels of IFN-γand IL-12 and IL-12 gene expression and the activation of NF-AT in peripheral mononuclear cells (PBMC) in patients of uveitis and investigate their relationship on uveitis Methods The levels of IFN-γand IL-12 were detected by ELISA; the IL-12mRNA was detected by situ hybridization(ISH)respectively; the activity of NF-AT was detected by electrophoretic mobility shift assay(EMSA). Result: The levels of IFN-γ?IL-12 and IL-12 gene expression and the activation of NF-AT in PBMC from the patients of uveitis were significance higher than normal contrast and other patients of ophthalmopathy (P<0.01).Conclusion: The abnormal expression of IL-12?IFN-γ and NF-AT, which is suggested that may contribute to the pathogenesis of uveitis .These results prompt that the imbalance of Th 1/Th 2 cell might exist in patients with uveitis. The significance higher of the regulation by NF-AT at transcription level may be associated with reinforcement transcription of IFN-γ in uveitis.

关 键 词：葡萄膜炎 患者 发病机制 外周血单核细胞 疾病相关细胞因子 细胞功能亢进 

分 类 号：R773.9[医药卫生—眼科]