基质金属蛋白酶、血管内皮细胞生长因子与角膜新生血管形成的相关性  

The Expression of MMP_2,VEGF in Inflammation -associated Corneal Neovascularization and Relational Study

作　　者：袁静[1] 王适群[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,湖北武汉430022 [2]美国密歇根州立大学微生物系

出　　处：《湖北民族学院学报（医学版）》2002年第4期7-10,共4页Journal of Hubei Minzu University(Medical Edition)

摘　　要：目的 研究大鼠角膜新生血管形成中,基质金属蛋白酶(MMP2)、血管内皮细胞生长因子(VEGF)的表达与炎性反应的相关性。方法 利用硝酸银烧灼角膜表面,建立鼠角膜新生血管模型,每日裂隙灯下观察角膜新生血管的发展,并于1、4、7、10、21d处死大鼠,摘取眼球作角膜病理切片,多形核白细胞(PMN)计数作统计分析;免疫组化方法分析MMP2、VEGF的表达。结果 光镜下显示正常组PMN计数为0.500±0.629,处理后第1d角膜缘(PMN)细胞开始增多,炎症后引起角膜新生血管(CNV)1d、4d、7d.10d、21d,PMN计数(每高倍视野均数)分别为14.200±1.723、19.200±2.401、28.700±1.632、15.100±2.097、0.700±0.816,以CNV7d组PMN增加最明显,此后减少;免疫组化显示:MMP2在CNV中阳性表达逐渐增加,于10d表达最明显,此后随炎性细胞的减少而减少,21d后几乎无表达;VEGF与MMP2的表达分布相似,但密度较弱。结论 炎症相关性角膜新生血管形成中,MMP2、VEGF的表达增加,并与角膜的炎性反应平行。Objective To study the expression of MMP2 and VEGF in inflammation associated corneal neovascularization and the correlation between the expression and inflammation. Methods Corneal neovascularization was induced by silver nitrate Cauterizing the surface of cornea, Slit - lamp biomicroscopy revealed a gradual development of corneal neovascularization. Animals were grouped and sacrificed at 24h,4d,7d, 10d,21d after CNV respectively. The corneas were enucleated by histopathological examination , PMNs were counted and statistical conclusions were given; the expression of MMP2, VEGF was detected by immuno-histochemistry. Results The histopathological study showed the number of PMNs were 0. 500 ±0. 629 in the untreated corneal group. The number of PMNs in CNV24h group,CNV4d group, CNV7d group, CNV10d group, CNV21d group were 14. 200 ±1. 723,19. 200 ±2. 401, 28.700±1.632, 15.100 ±2. 097, 0. 700 ±0. 816 respectively. In CNV7d group, PMN increased superlatively and began to decrease 7d after . Immunochemistry demonstrated: the positive expression of MMP2 in CNV progressed gradually, and involved the entire cornea 10 days post-treatment. After 10 days the expression decreased following the decreaseof inflammatory cells, 20 days post -treatment the positive expression was not shown. The positive expression of VEGF was paralleled by the expression of MMP2, but expressed weaker than the latter. Conclusions In inflammation -associated corneal neovascularization, expression of MMP2, VEGF progressed and paralleled by the corneal inflammatory reaction.

关 键 词：基质金属蛋白酶 血管内皮细胞生长因子 大鼠 角膜新生血管形成 炎性反应 相关性 

分 类 号：R772.21[医药卫生—眼科]