猪苓多糖对膀胱癌细胞癌基因蛋白表达的影响  

The Expression of P53 and H-ras in Bladder Cancer Cells Induced by Polyporus Polysaccharide

作　　者：曾星 章国来 梅玉屏 黄羽 何盛琪 

机构地区：[1]广州中医药大学第二附属医院,广州市510120

出　　处：《中国肿瘤临床》2003年第2期81-83,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:39870944);高等学校优秀教师基金资助(2001)39

摘　　要：目的:分析猪苓多糖对体外培养T24膀胱癌细胞癌基因蛋白表达的影响。方法:应用免疫荧光法、激光共聚焦显微镜对p53、H-ras基因蛋白表达进行定位、定量观察。结果:猪苓多糖显著增加p53蛋白表达,24小时表达最高,呈弥漫性分布,随后逐渐下降;对照组在24小时表达很弱,48小时表达最强。猪苓多糖对H-ras基因蛋白表达无明显影响。结论:猪苓多糖对T24细胞p53基因蛋白表达有一定的调节作用,对H-ras基因蛋白表达无明显影响。Objective:To elucidate the specific mechanisms of the expression of P53and H-ras in bladder cancer cells induced by polyporus polysaccharide(PPS).Methods:Laser scan-ning confocal microscope was used to investigate the distribution of the p53?H-ras protein in t24bladder cancer cells.Results:p53labeled with fluorescence spread all over the cytosol and nuclei in the T24cells after24h treatment with PPS,while the fluorescent intensity decreased after48h stimulation;No correlation with expression of H-ras in T24cells was found.Conclu-sion:These results suggested enhanced expression of P53of bladder cancer cells induced by PPS.

关 键 词：膀胱癌 猪苓多糖 癌基因 免疫荧光法 激光共聚焦显微镜 p53基因 H-RAS基因 蛋白表达 

分 类 号：R737.14[医药卫生—肿瘤]