lunx mRNA RT-PCR检测肺癌的微转移  

Detection of Micrometastasis of Lung Cancer by Using Lunx mRNA Specific Reverse Transcription-polymerase Chain Reaction

作　　者：朱广迎[1] 刘德林[2] 王绪[2] 彭猛青[2] 陈杰[2] 贾晓民[2] 

机构地区：[1]北京大学临床肿瘤学院放疗科 [2]徐州医学院肿瘤学教研室,江苏省徐州市221002

出　　处：《中国肿瘤临床》2003年第2期124-127,共4页Chinese Journal of Clinical Oncology

基　　金：江苏省卫生厅科研基金资助(编号:Z9709)

摘　　要：目的:建立以lunx为靶基因,RT-PCR检测肺癌患者区域淋巴结和外周血中微转移的方法,检验lunxmRNA作为肺癌微转移检测分子标志物的可行性。方法:以肺部良性疾病30例、健康人10例外周血为对照,采用逆转录PCR(reversetranscriptasepolymerasechainreaction,RT-PCR)法半定量检测lunxmRNA的表达,研究26例肺癌患者外周血微转移情况;以因肺良性疾病切除的11枚淋巴结为对照,采用RT-PCR法半定量检测lunxmRNA表达研究肺癌手术切除区域淋巴结44枚微转移情况并与常规病理对比。结果:20例非小细胞肺癌(non-smallcelllungcancer,NSCLC)外周血lunxmRNA阳性率为60.0%,6例SCLC中4例(67.0%)外周血lunxmRNA阳性。在44枚肺癌患者区域淋巴结中16枚(36.4%)表达lunxmRNA,高于常规病理的13.6%(P<0.05);而30例肺良性疾病和10例健康者外周血以及11枚正常淋巴结中均不表达lunxmRNA。结论:lunxmRNART-PCR法检测肺癌患者外周血和手术切除区域淋巴结微转移具有较高的特异性和敏感性,有助于指导临床治疗。Objective:Detection of lung cancer micrometastases in peripheral blood and re-gional lymphatic nodes by using lunx mRNA specific reverse transcription-polymerase chain reac-tion(RT-PCR).Methods:RT-PCR was used to check-up lunx mRNA in peripheral blood of26patients with lung cancer semiquantitatively,compared to30patients with benign lung disease and10healthy volunteer.Lunx mRNA of44regional lymphatic nodes obtained from25patients with lung cancer underwent curative lobectomy was checked-up with RT-PCR.The results were compared to that of regular histopathology.Results:The positive rate of lunx mRNA in peripheral blood for non-small cell lung cancer(NSCLC)and small cell lung cancer(SCLC)patients were60%(12/20)and67%(4/6)respectively.The positive rate of lunx mRNA of lymphatic nodes of patients with lung cancer is36.4%,higher than that of regular histopathology(χ 2 =6.06,P<0.05).However,no blood samples and lymphatic nodes from pulmonary benign diseases patients or nor-mal volunteers were positive for lunx mRNA.Conclusion:RT-PCR amplification of lunx mRNA is an efficient and specific mean to detect early haematogenous and regional lymphatic nodes dis-semination of cancer cells for patients with lung cancer.

关 键 词：肺癌 lunx基因 RT-PCR检测 淋巴结微转移 外周血微转移 微转移检测分子标志物 可行性 

分 类 号：R734.2[医药卫生—肿瘤]