胃粘膜线粒体DNA核内整合与IL-8活性的关系  

Relationship between integration of mitochondrial DNA( mtDNA)in the nuclei of gastric mucosa and IL-8 activity.

作　　者：凌贤龙[1] 房殿春[1] 杨仕明[1] 汪荣泉[1] 杨建民[1] 刘海峰[1] 彭贵勇[1] 刘为纹[1] 

机构地区：[1]第三军医大学西南医院全军消化专科中心,重庆400038

出　　处：《重庆医学》2003年第2期171-172,共2页Chongqing medicine

基　　金：国家自然科学基金资助项目 (30 0 70 0 4 3) ;全军"十五"重点基金资助 (0 1Z0 75)

摘　　要：目的　评价胃粘膜线粒体DNA核内整合与组织IL 8活性的关系。方法　采用原位杂交方法检测线粒体DNA核内整合 ;组织IL 8含量测定采用ELISA方法。结果　胃癌细胞核内mtDNA序列的检出率为 2 0 % (6 /30 )、异性增生为 10 % (1/10 )、肠上皮化生粘膜为 6 .7% (1/15 )、萎缩性胃炎为 10 % (1/10 ) ,浅表性胃炎未发现有mtDNA序列整合。胃粘膜细胞IL 8活性在mtDNA序列的检出组显著高于mtDNA序列非检出组 (P <0 .0 5 )。结论　胃粘膜细胞mtDNA序列整合可能参与了胃癌的发生 ;胃粘膜细胞mtDNA序列核内整合可能与IL 8活性有关。Objective To evaluate the relationship between integration of mitochondrial DNA( mtDNA)in the nuclei of gastric mucosa and IL 8 activity. Methods The sequences of mtDNA in the nuclei was detected in situ hybridization. IL 8 level in gastric mucosa was examined with ELISA method. Results The integration of mtDNA in the nuclei was detected in 1/10(10%) chronic atrophic gastritis,1/15(6.7%)intestinal metaplasia,1/10(10%)dysplasia,6/30(20%) gastric cancer. The IL 8 level in the group with integration of mtDNA in the nuclei was significantly higher than that in the group without integration of mtDNA in the nuclei ( P <0.05). Conclusions The integration of mtDNA into nuclei of gastric mucosa may be involved in the carcinogenesis of gastric mucosa and IL 8 may contribute to the integration of mtDNA into nuclei of gastric mucosal cells.

关 键 词：胃粘膜线粒体 DNA核内整合 IL-8活性 原位杂交 胃癌细胞核 

分 类 号：R573[医药卫生—消化系统]