羊膜对培养的人视网膜色素上皮细胞增殖影响的实验研究  

An experimental study of the effects of human amniotic membrane on human retinal pigment epithelial cell proliferation in vitro

作　　者：李世洋[1] 王一[1] 

机构地区：[1]第三军医大学附属西南医院眼科,重庆400038

出　　处：《第三军医大学学报》2003年第5期407-409,共3页Journal of Third Military Medical University

摘　　要：目的　观察羊膜是否对视网膜色素上皮细胞 (Retinalpigmentepithelial ,RPE)的生长有影响 ,探讨羊膜用于治疗增殖性玻璃体视网膜病变 (Proliferativevitreoretinopathy ,PVR)的潜在可能性。方法　运用MTT比色法 ,观察羊膜匀浆在不同浓度 (4 0、80、160 μg ml)及不同作用时相点 (2 4、48、96h)对人RPE增殖的影响。 结果　①在第 2 4小时 ,3个浓度羊膜匀浆均对人RPE增殖起抑制作用 ,而且随浓度增加抑制作用减弱 ,抑制率 (5 0 774%、2 7 3 87%、14 80 2 % )间相差显著 (P <0 0 5 ) ;在第 48、96小时 ,羊膜匀浆在低浓度时 ,对人RPE增殖起抑制作用 ,而在中、高浓度为促增殖作用 ,其中在第 48小时抑制率 (4 494%、-0 944 %、-3 693 % )间相差不显著 (P >0 0 5 ) ,在第 96小时时抑制率 (8 3 88%、-9 42 5 %、-17 65 1% )间相差显著 (P <0 0 5 )。②羊膜匀浆浓度为 40 μg ml ,各时相点对人RPE均为抑制增殖 ,抑制率间相差非常显著 (P <0 0 1) ;在浓度为 80 μg ml、160 μg ml时 ,随着作用时间延长 ,羊膜匀浆对人RPE增殖的作用逐渐由抑制变为促进作用 ,抑制率间相差显著 (P <0 0 5 )。结论　羊膜匀浆在低浓度 ,短时间内对人RPE增殖有抑制作用 ,而在高浓度、长时间则对RPE增殖有促进作用。羊膜匀浆用?Objective To observe the effects of human amniotic membrane (HAM) on human retinal pigment epithelial (HRPE) cells proliferation so as to find a new treatment method for proliferative vitreoretinopathy (PVR). Methods We observed the effects of supernatant of homogenized fresh amnion(SHFA) of different concentrations on HRPE cells proliferation at different time points by tetrazotium (MTT) colorimetric assay. Results (1) The proliferation of HRPE cells was inhibited at 24 h after being cultured with SHFA. The effect of inhibition decreased with the increasing concentration of SHFA in DME/Ham's F12(D/F12). There was significant difference of inhibition rate among the groups (50 774%, 27 387% and 14 802%)( P <0 05). At 48 and 96 hours after culture, the proliferation of HRPE cells was inhibited in 40 μg/ml groups, but could be promoted in 80 μg/ml and 160 μg/ml groups. However, there was no significant difference of inhibition rate among the groups (4 494%, -0 944% and -3 693%) ( P >0 05). At 96 hours after culture, the difference of inhibition rate among the groups(8 388%, -9 425% and -17 651%) was significant ( P <0 05). (2) In 40μg/ml concentration group, the proliferation of HRPE cells was inhibited at 24 h, 48 h and 96 h after culture. The difference of the inhibition rate between 24 h and 48 h, between 24 h and 96 h was significant ( P <0 01). In 80 μg/ml and 160 μg/ml groups, the proliferation of HRPE cells was inhibited at 24 h, but promoted at 48 h, 96 h after culture. The difference of the inhibition rate between 24 h and 96 h ( P <0 01), between 24 h and 48 h ( P <0 05) was significant. Conclusion The proliferation of HRPE cells can be inhibited by SHFA of lower concentration (40 μg/ml) within shorter period (24 h), but be promoted by SHFA of higher concentration (80 μg/ml, 160 μg/ml) within longer period(48 h～96 h), suggesting there is little possibility that PVR can be treated with SHFA.

关 键 词：羊膜 视网膜色素上皮细胞 细胞增殖 增殖性玻璃体视网膜病变 孔源性视网膜脱离 

分 类 号：R774.12[医药卫生—眼科] R739.72[医药卫生—临床医学]