苯丁酸氮芥诱导Behcet病患者活化淋巴细胞凋亡的研究  

The induction of apoptosis by chlorambucil on activated lymphocytes from the patients with Behcet' s disease.

作　　者：张震[1] 杨培增[1] 王红[1] 周红颜[1] 黄翔坤[1] 李芬芳[1] 

机构地区：[1]中山大学中山眼科中心,广州510060

出　　处：《眼科研究》2003年第1期1-3,共3页Chinese Ophthalmic Research

基　　金：国家杰出青年基金(39925034);跨世纪优秀人才基金(敦技函[1999]2号)资助

摘　　要：目的 研究苯丁酸氮芥对Behcet病患者活化淋巴细胞凋亡的诱导作用。方法 分离正常人与Behcel病患者外周血淋巴细胞并用PHA刺激活化,然后加入苯丁酸氮芥进行体外培养,利用Annexin-V/PI双标记法结合流式细胞技术定量检测淋巴细胞的凋亡。 结果 (1)正常人和Behcet病患者外周血淋巴细胞经PHA活化后均可发生凋亡,其自发凋亡率分别为20.9％±3.4％和16.3％±2.7％,二者具有显著性差异(t=2.36,P<0.05);(2)Behcet病患者的活化淋巴细胞经苯丁酸氮芥作用后凋亡细胞均显著增多,凋亡率从16.3％±2.7％上升至23.6％±5.6％(t=2.72,P<0.05)。结论 Behcet病患者的活化淋巴细胞对凋亡具有抵抗性;苯丁酸氮芥可诱导Behcet病患者的活化淋巴细胞发生凋亡,此可能是其控制炎症与防止炎症复发的作用机制之一。Objective To study the induction of apoptosis by chloramburil on activated peripheral lymphocytes from patients with Behcet ' s disease. Methods Peripheral lymphocytes obtained from 10 healthy donors and 15 patients with Behcet' s disease were stimulated with PHA and then cultured with the presence of chlorambucil. The apoptotic lymphocytes were labeled with annexin-V/propidium iodide and measured using flow cytometry. Results ( 1 ) Apoptosis were observed in the lymphocytes stimulated with PHA both from healthy donors ( 20. 9% ± 3. 4% ) and patients with Behcet' s disease ( 16. 3% ± 2. 7% ). But the apoptotic rale was significantly different ( t = 2. 36 , P < 0. 05 ) between patients and healthy donors. ( 2 ) The percentage of the apoptotic lymphocytes from the patients was significantly increased from 16.3% ±2.7% to 23.6% ±5.6% after treatment with ehlorambucil (t = 2. 72 , P < 0. 05 ) . Conclusion Activated lymphocytes from the patients with Behcet' s disease are relatively resistant to apoptosis. Chlorambucil can effectively induce activated lymphocytes of the patient with Behcet' s disease into apoptosis .which may be one of the effective mechanisms for ehlorambucil to control the intraocular inflammation.

关 键 词：苯丁酸氮芥 BEHCET病 淋巴细胞 细胞凋亡 顽固性葡萄膜炎 

分 类 号：R773.9[医药卫生—眼科]