人类新的肝癌高表达基因HCCA3的克隆、鉴定及其临床病理学意义  

Cloning and identification of a novel gene HCCA3 highly expressed in human hepatocellular carcinoma and its clinicopathological significance

作　　者：洪毅[1] 曾锦章[1] 傅骁勇[1] 郭婷婷[1] 邱秀华[1] 王征旭[1] 刘淑琴[1] 吴孟超[1] 王红阳[1] 

机构地区：[1]第二军医大学东方肝胆外科医院第二军医大学国际合作生物信号转导研究中心,上海200438

出　　处：《中华实验外科杂志》2003年第3期216-218,共3页Chinese Journal of Experimental Surgery

基　　金：国家 973项目 (G1 9980 51 2 1 0 );国家自然科学基金资助项目 (39830 0 80;3982 51 1 4;30 0 70 833)

摘　　要：目的　克隆新的肝癌相关基因并探索其在肝癌发生发展中的作用机制。方法　采用mRNA差异显示技术 ,分析肝癌和癌旁组织差异表达基因谱 ,获得的差异表达基因片断作为探针 ,筛选胎盘cDNA文库 ,以获得全长cDNA序列。通过Northern印迹杂交的方法 ,分析所获得的新基因在 42例肝癌组织中的表达情况 ,并分析其临床和病理学意义。结果　克隆了一个新的肝癌相关基因 ,命名为HCCA3 ,该基因的全长cDNA 170 6bp ,编码一个 2 6 4个氨基酸的蛋白质 ,在人类肝癌中广泛表达 ( 78.6 % ,33/42 ) ,并与肝癌的浸润转移密切相关。结论　HCCA3是一个新的人类基因 ,在肝癌中的异常高表达可能对肝癌的发生发展具有重要的促进作用。Objective To clone novel hepatocellular carcinoma (HCC) associated genes that may provide new insight to molecular mechanisms of this disease development.Methods DD-PCR was employed to analyze altered gene expression patterns between HCC tissues and their adjacent non-tumor tissues.The cloned PCR fragment served as probe to screen a human placental cDNA library to obtain the full-length cDNA.mRNA expression level of the isolated gene were detected by Northern blotting in 42 patients with HCC and its clinopathological significance was analyzed.Results A novel HCC-associated gene named HCCA3 with a transcript of 1706 bp in length that encodes a protein of 264 amino acids was identified.Northern blot analysis revealed that HCCA3 mRNA was highly expressed in HCCs (78.6%,33/42) and significantly correlated with HCC metastasis.Conclusion HCCA3 is highly involved in HCC development.

关 键 词：肝癌 高表达基因 HCCA3 克隆 鉴定 基因表达 MRNA 

分 类 号：R735.7[医药卫生—肿瘤]