大鼠注射戊四氮后海马突触可塑性的研究  

Synaptic plastisity in hippocampus in epileptogenesis of pentylenetrazol kindling rats

作　　者：朱遂强[1] 雷霆[2] 杨嘉君[1] 张梁[1] 王开颜[1] 阮旭中[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030 [2]华中科技大学同济医学院附属同济医院神经外科,武汉430030

出　　处：《中华实验外科杂志》2003年第3期265-266,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然基金资助项目 (30 1 70 333)

摘　　要：目的　探讨戊四氮 (PTZ)点燃过程中电镜下大鼠海马突触的可塑性。方法　大鼠随机分为对照组和药物组PTZ 35mg/kg ,腹腔注射 ,每日 1次 ) ,利用透射电子显微镜观察拍片 ,作图像分析大鼠海马突触密度和突触类型的改变。结果　药物组大鼠注射戊四氮后在行为学未出现惊厥 ,脑电图未出现痫性放电的点燃前潜伏期内 ,出现大鼠海马突触密度明显增加 (PTZ4d突触密度为 9.5 5± 0 .37、PTZ 10d为 11.43± 0 .48、PTZ 14d为 11.99± 0 .6 7) ,与对照组 (突触密度 7.31±0 .6 5 )比较 ,差异有显著性 (P <0 .0 5 ) ;药物组大鼠重建的突触以Ⅰ型突触为主。结论　大鼠注射戊四氮后引起海马突触数量和类型的改变 ,可能与形成异常神经元放电环路 ,最终诱发癫痫发作有关。Objective To investigate the synaptic plastisity in hippocampus in epileptogenesis of pentylenetrazol kindling rats under electron microscopy.Methods The rats were randomly divided into control group and experimental group [Pentylenetetrazol (PTZ),35 mg/kg,intraperitoneal injection,once daily].Under electron microscopy,the changes of synaptic density and synaptic types in hippocampus were observed.Results During PTZ-kindling of rats before the occurrence of spontaneous seizure confirmed by behaviors and EEG,synaptic density in hippocampuswas significantly increased (9.55±0.37,11.43±0.48 and 11.99±0.67 respectively at 4th day,10th day and 14th day) as compared with that in control group (7.31±0.65) (P<0.05).Most of synapses were type Ⅰ in the experimental group.Conclusion The injecticn of PTZ can cause the changes of synaptic number and type in hippocampus,which may be responsible for the abnormal discharge circuit,then induction of the seizures.

关 键 词：大鼠 注射 戊四氮 海马突触 可塑性 研究 脑电图 透射电子显微镜 

分 类 号：R742.1[医药卫生—神经病学与精神病学]