抗人卵巢癌单链抗体噬菌体表面呈现文库的构建、初步筛选及鉴定  

Construction and preliminary selection and identification of phage display library of anti-human ovarian cancer single chain Fv antibody

作　　者：杜辉[1] 王建[1] 辛晓燕[1] 郑维国[1] 

机构地区：[1]第四军医大学西京医院妇产科,陕西西安710033

出　　处：《第四军医大学学报》2003年第6期536-539,共4页Journal of the Fourth Military Medical University

摘　　要：目的 :构建抗人卵巢癌全套单链抗体基因噬菌体表面呈现文库 .方法 :利用噬菌体表面呈现技术 ,构建基因文库 ,经过panning筛选富集后 ,用ELISA方法检验抗原结合活性 .结果 :从单个噬菌体克隆中筛选到 2 6个具有抗原结合活性的阳性克隆 .结论AIM: To construct the phage display library of anti human ovarian cancer single chain Fv antibody. METHODS: The ScFv gene library was constructed by phage display technology. The affinity to antigen was detected by ELISA after panning. RESULTS: Twenty six antigen binding clones were identified from 94 individual phagemid clones. CONCLUSION: In the production of engineering antibodies against human cancer, this strategy may provide an alternative approach.

关 键 词：噬菌体表面呈现 单链抗体 卵巢肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]