人肺癌中OPB7-1 cDNA片段的克隆定位  

Cloning and mapping analysis of cDNA fragment OPB7-1 gene in human lung adenocarcinoma

作　　者：樊红[1] 李钰[1] 邓艳秋[2] 陈英准[2] 冯会臣[1] 傅松滨[1] 张贵寅[1] 李璞[1] 

机构地区：[1]哈尔滨医科大学遗传教研室,150086 [2]哈尔滨医科大学病理教研室,150086

出　　处：《中华医学遗传学杂志》2003年第2期156-159,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金重大项目 (39392 90 0 ) ;国家自然科学基金 (39970 396)~~

摘　　要：目的　寻找肺腺癌发生及转移的相关基因 ,探讨肺癌发生发展的分子机理。方法　应用细胞培养、逆转录 PCR、RH基因定位及RNA原位杂交方法研究确定肺癌相关基因。结果　OPB7 1cDNA片段 ,经RH基因定位方法定位于 1p3 1 1p3 4。在正常人肺cDNA文库、低转移能力肺腺癌细胞系AGZY83 acDNA中存在的片段大小一致 ,但序列中存在个别碱基的差别。OPB7 1cDNA片段 ( 974bp)与GenBank中已知序列同源性差 ,但与其有同源性的 3个毗连群克隆均位于 1号染色体上 ( 1p3 1 1p3 4)。 2 4例临床病例分析发现 ,该片段在肺癌组织中均有不同程度的表达 ,在正常对照组织中未见明显表达 ,且在有淋巴结转移病例的肺癌组织中表达程度呈明显增高的趋势。结论　提示OPB7 1可能为新的基因 ,与肺癌的发生、转移可能有一定的相关性。Objective: To search the candidate gene in the development and metastasis of lung adenocarcinoma and shed light on the possible molecular mechanism of the development of lung carcinoma. Methods: Using methods of cell culture, reverse transcription-PCR, RH gene mapping and RNA in situ hybridization. Results: The cDNA fragment named OPB7-1 was mapped at 1p31-1p34 by RH gene mapping method. The fragment sequences obtained from lung cDNA library of normal person and cell line of AGZY83-a were similar in length but showed individual base difference. For OPB7-1, there is a low homogeneity to known gene by analysis in GenBank, but 3 contigs homologous to OPB7-1 were located at chromosome 1(1p31-1p34). Different degrees of expression were noted in tumor tissues from 24 cases of lung carcinoma, however no significant expression was found in their corresponding normal tissues. And high expression was found in the lung tissues of cases with lymph node metastasis. Conclusion: OPB7-1 may be a novel gene. It may be a tumor related gene in occurrence and metastasis of lung carcinoma.

关 键 词：肺腺癌 RH基因定位 RNA原位杂交 基因表达 

分 类 号：R734.2[医药卫生—肿瘤]