α_5β_1在舌鳞状细胞癌中的表达及其在细胞粘附与运动中的作用  

Expression of α_5β_1 in Tca-8113 cells and its roles in cellular adhension and migration

作　　者：赵玲[1] 叶志佳[1] 唐毅[2] 

机构地区：[1]广州市红十字会医院口腔科,510220 [2]暨南大学医学院第四附属医院

出　　处：《现代口腔医学杂志》2003年第2期116-119,共4页Journal of Modern Stomatology

摘　　要：目的　探讨舌鳞状细胞癌Tca - 8113细胞纤维连接蛋白受体α5β1的表达 ,及α5β1在Tca - 8113细胞粘附、运动中的作用。方法　培养Tca- 8113细胞 ,采用间接免疫荧光检测细胞α5β1的表达 ;应用抗α5β1抗体结合细胞表面纤维连接蛋白受体α5β1,分析α5β1在细胞粘附、运动行为中的作用。结果　α5β1在舌鳞状细胞癌Tca -8113细胞表面呈点状和团块状分布 ;抗α5β1抗体可以显著抑制细胞在纤维连接包被表面的粘附和运动行为。结论　舌鳞状细胞癌细胞存在α5β1的表达 ,α5β1在舌鳞状细胞癌的浸润、转移中可能起着重要的作用。ObjectiveTo investigate the expression of α 5β 1 in Tca-8113 cell line of tongue squamous cell carcinoma and its roles in adhension and migration of the cells.MethodsTca-8113 cells were cultured with RPMI-1640 medium in vitro.Expression of α 5β 1 in Tca-8113 cells was detected by indirect immunoinfluorescence.Incubated with rabbit anti-human α 5β 1 antibody,the adhesion and migration of the cells on fibronectin coated surface were analyzed.ResultsTca-8113 cells could produce α 5β 1 and showed brightly dot or punctate staining over entire cellular surface when examined by immunofluorescence with antibody to α 5β 1.Antibody to α 5β 1 could significantly decrease the adhesion and migration of Tca-8113 cells on fibronectin coated surface.ConclusionTca-8113 cells can synthesize α 5β 1 on the cellular surfaces,and α 5β 1 may play an important role in the invasion and metastasis of tongue squamous cell carcinoma.

关 键 词：纤维连接蛋白 舌鳞状细胞癌 α5β1 粘附 运动 

分 类 号：R739.8[医药卫生—肿瘤]