兔肝动脉化疗栓塞术实验方法学的对比性研究  

Experimental Methodology of Transhepatic Arterial Chemoembolization in Rabbits: A Comparative Study

作　　者：孔健 冯敢生[1] 许林锋[1] 梁惠明 郑传胜[1] 吴汉平[1] 柳曦[1] 李欣[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院放射科

出　　处：《临床放射学杂志》2003年第3期244-247,共4页Journal of Clinical Radiology

摘　　要：目的　探讨兔肝动脉化疗栓塞的实验方法。材料与方法　大耳白兔 168只 ,随机分为股动脉插管组(n =2 0 )及剖腹下直视穿刺组 (n =148) ,比较两种实验方法的手术及X线下操作时间、手术成功率、动物存活率。结果　股动脉插管组平均手术操作时间 145 .4± 2 4.6min ,X线透视时间 44 .3± 10 .7min ,手术成功率为 2 0 % ( 4/ 2 0 ) ,动物在术后 3天内均死亡。剖腹直视下穿刺组平均手术操作时间为 49.8± 12 .7min ,X线透视时间 7.9± 4.3min ,手术成功率为 97.3 % ( 144 / 148) ,长期存活率为 91.2 % ( 13 5 / 148)。经统计学检验 ,两者间有极显著性差异 (P <0 .0 1)。结论　直视下兔肝动脉细针穿刺行化疗栓塞术是一种简便。Objective To discuss the experimental methodology of transhepatic arterial chemoembolization in rabbits.Materials and Methods One hundred and sixty-eight rabbits were randomly divided into study group (n=148) and control group (n=20). At laparotomy under direct vision, direct fine needle puncturing catheterization of hepatic artery was performed in study group, and transfemoral catheterization technique was used in control group. The operative time, fluoroscopic time, technical successful rate and the survival of the rabbits in two groups were compared. Results In the study group, the mean operative time, mean fluoroscopic time and technical successful rate were 49.8±12.7 minutes, 7.9±4.3 minutes and 97.3% (144/148), respectively, with a long-term survival rate of 91.2% (135/148), while in the control group, the results were 145.4± 24.6 minutes, 44.3±10.7 minutes and 20% (4/20), respectively, and all rabbits died within 3 days after the surgery. Significant difference in the above four respects existed between study and control group (P<0.01).Conclusion At laparotomy under direct vision, direct fine needle puncturing catheterization of hepatic artery is an effective and simple procedure for experimental hepatic chemoembolization in rabbits.

关 键 词：肝动脉化疗栓塞术 实验方法学 对比性 动物实验 

分 类 号：R730.5[医药卫生—肿瘤]