益胃冲剂对胃癌前病变大鼠粘膜上皮细胞DNA量及AgNOR数的影响  

Effects of YiWeiChongJi on DNA content and AgNOR counting in epithelial cells of gastric mucosa in rats with precancerous lesion of gastric carcinoma

作　　者：王文[1] 夏天[1] 张仲海[1] 王宗仁[1] 张金洲[2] 李锋[1] 李军昌[1] 行利[1] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710033 [2]第四军医大学西京医院心血管外科,陕西西安710033

出　　处：《第四军医大学学报》2003年第5期409-411,共3页Journal of the Fourth Military Medical University

基　　金：陕西省中医管理局科研课题 (0 32 )

摘　　要：目的 :从实验角度对益胃冲剂的药效进行观察 ,并对其逆转机制加以探讨 .方法 :5 0只SD大鼠随机分为正常组、模型组、益胃冲剂高、低剂量治疗组、维酶素对照组 ,分别对各组病理变化 ,胃粘膜上皮细胞AgNOR计数 ,DNA相对含量及DNA相对倍体值进行观察 .结果 :模型组大鼠 87.5 %出现粘膜变薄 ,腺体萎缩 ,10 0 %出现炎细胞浸润 ,6 6 .5 %出现肠上皮化生 ,37.5 %出现异型增生 ,其粘膜上皮细胞AgNOR计数 ,DNA相对含量及DNA相对倍体值明显增高 ,而治疗组粘膜变薄、腺体萎缩 ,炎细胞侵润 ,肠上皮化生 ,不典型增生的发生率分别为高剂量组 :11.1% ,11.1% ,11.1% ,0 ,0和低剂量组 :2 2 .5 % ,11.1% ,2 2 .2 % ,11.1% ,0较模型组的 87.5 % ,87.5 % ,10 0 % ,6 2 .5 % ,37.5 %明显改善 (P <0 .0 1) .AgNOR计数、DNA相对含量及相对倍体值较模型组明显降低 ,与维酶素对照组存在显著差异 (P <0 .0 5 ) .结论 :益胃冲剂能有效地逆转胃粘膜癌前病变 ,其逆转机制可能与其降低病变细胞DNA相对含量。AIM: To study the curative effects and the mechanism of YiWeiChongJi. METHODS: 50 rats were divided randomly and evenly into 5 groups: Normal group, Model group, Therapy group with high dosage of YiWeiChongJi, Therapy group with low dosage of YiWeiChongJi and Control group with WeiMeiSu. The pathological changes, AgNOR counting, DNA relative content and U levels of epithelial cells of gastric mucosa in each group were examined. RESULTS: In Model group, mucosa got thin and gland got atrophied in 87.5% of the rats, infiltration of inflammation cells presented in 100%. Intestinal metaplasia was seen in 66.5% of the rats and atypical hyperplasia in 37.5%. The AgNOR counting, DNA content and U level of epithelial cells increased markedly. However, in the Therapy group with low or high dosage of YiWeiChongJi, pathological changes ameliorated significantly ( P <0.01). Compared with those of the Model group, the AgNOR counting and DNA content, U level of epithelial cells declined markedly, which were statistically different from those of the Control group ( P <0.05). CONCLUSION: YiWeiChongJi can effectively reverse the precancerous lesions in gastric carcinoma and its mechanism of reversion probably relates to the reduction of DNA content and restraint of abnormal active hyperplasia.

关 键 词：AGNOR 益胃冲剂 胃癌前病变 DNA 中医药疗法 

分 类 号：R735.2[医药卫生—肿瘤] R273.52[医药卫生—临床医学]