百会穴在电针刺激预处理诱导脑缺血耐受中的作用  

Baihui electroacupuncture in the induction of brain ischemic tolerance by preconditioning

作　　者：劳宁[1] 熊利泽[1] 路志红[1] 刘艳红[1] 郑玉[1] 巩固[1] 

机构地区：[1]第四军医大学西京医院麻醉科,陕西西安710033

出　　处：《第四军医大学学报》2003年第5期418-420,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金 (30 1 70 90 7)

摘　　要：目的 :观察电针刺激对大鼠局灶性缺血 /再灌注损伤的预处理效应的穴位特异性 .方法 :SD雄性大鼠 4 0只随机分为 4组 (n =10 ) ,即空白对照组 (Control)、戊巴比妥对照组(PB)、针刺百会穴组 (EA1)和针刺穴位旁开组 (EA2 ) .Control组未行任何处理 ;PB组腹腔注射戊巴比妥钠 4 0mg·kg-1·d-1,连续 5d;针刺EA1组和EA2组在戊巴比妥钠麻醉下分别用重复电针刺激 (30min·d-1× 5d)百会穴和百会穴右旁开 1.0cm处 .最后一次处理的 2 4h后 ,所有动物在异氟醚麻醉下用颈内动脉线栓法致右侧大脑中动脉栓塞 12 0min .再灌注 2 4h后 ,行神经功能障碍评分 ,并取大脑行TTC染色以测量脑梗死容积 .结果 :①神经功能障碍评分EA1组的评分优于Control组和PB组 (P <0 .0 5 ) ,与EA2组比较无差异 .EA2组、Control组和PB组的评分无显著差异 ;②脑梗死容积EA1组的脑梗死容积较其他 3组显著减少 (P <0 .0 1) .EA2组的梗死容积较Control组和PB组显著减小 (P <0 .0 1) .Control和PB组间的梗死容积无差异 (P >0 .0 5 ) .结论 :重复电针刺激预处理对大鼠局灶性脑缺血 /再灌注损伤的预处理效应有穴位特异性 .AIM: To investigate the acupoint specificity of brain ischemic tolerance induced by preconditioning with repeated electroacupuncture at 'Baihui' acupoint in rats with transient focal cerebral ischemia. METHODS: 40 SD male rats were randomly divided into 4 groups ( n =10 each): Animals in control group received no pretreatment, animals in PB group were intraperitoneally injected with pentobarbital sodium 40 mg/kg a day for 5 days, animals in EA1 group received electroacupuncture at the 'Baihui' acupiont 30 min a day for 5 days under pentobarbital sodium anesthesia and animals in EA2 group received electroacupuncture at the point 1.0 cm right from 'Baihui' acupoint 30 min a day for 5 days under pentobarbital sodium anesthesia. 24h after the last treatment, the right middle cerebral artery occlusion model (MCAO) was induced for 120 min in all animals. The neurological deficit scores (NDS) were evaluated at 24h after reperfusion and then rats were sacrificed under deep anesthesia. The brain was cut into 6 2 mm-thick coronal sections and infarct volumes were determined by TTC staining. RESULTS: ① EA1 group showed lower NDS than that in control and PB groups ( P <0.05). There was no significant difference of NDS among the other groups. ② The infarct volume of EA1 group was smaller than that in any other groups ( P <0.01). The infarct volume of EA2 group was smaller compared with that in control or PB groups ( P < 0.01). No significant difference was observed between control and PB groups ( P >0.05). CONCLUSION: The ischemic tolerance induced by preconditioning with repeated electroacupuncture at preconditioning in rats with focal cerebral ischemia has acupoint specificity.

关 键 词：百会穴 电针 脑动脉栓塞 

分 类 号：R743.33[医药卫生—神经病学与精神病学]