L1210克隆细胞mRNA的表达及蛋白电泳图谱分析  

The Expression of mRNA and SDS-PAGE of L1210 Cell Strains and Its Cloned Cells

作　　者：刘秋燕[1] 吕占军[1] 王秀芳[1] 刘福英[1] 

机构地区：[1]河北医科大学实验动物学部,石家庄050017

出　　处：《四川大学学报（医学版）》2003年第2期207-209,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家"九五"攻关课题 ( 96-A2 3 -0 6-0 3 )资助

摘　　要：目的　研究 L12 10及其克隆细胞 m RNA表达的差异 ,从基因水平探讨质控瘤株的方法。方法　用SDS- PAGE电泳观察瘤细胞株的蛋白质电泳图谱 ;6株生物素标记的探针与瘤细胞进行玻片原位杂交测定瘤细胞m RNA的表达。结果　北京肿瘤所 L12 10蛋白质条带数为 32条带 ,克隆细胞 L3F9和 L3E11均为 31条带 ;细胞原位杂交证明 ,北京肿瘤所 L12 10与 6株探针均杂交阳性 ,但杂交阳性染色强度不同 ;克隆细胞 L3E11与 p16、c- jun及 p5 3探针杂交阳性 ;克隆细胞 L3F9与 p16 ,c- fos,c- myc及 c- jun探针杂交阳性。结论　从北京肿瘤所保存的L12 10细胞中获得的 2株克隆细胞 L3E11和 L3F9的蛋白质电泳图谱的条带数一致 ,但其 m RNA的表达不同 ;c-fos,c- myc以及 p5 3基因的表达与否可以把这 2株克隆细胞区别开来 ,cObjective To examine the expression difference of mRNA of L1210 cell strains and its cloned cells and discuss the methods for quality control of cell strains. Methods We used SDS PAGE to observe the difference of protein and performed in situ hybridization to examine the expression of mRNA with the use of 6 cDNA probes that were marked by biotin. Results The number of protein bands of L1210 from Beijing Cancer Institute was 32. The number of protein bands of the two cloned cells L3E11 and L3F9 was 31. The 6 cDNA probes (p16, c fos, c jun, c myc, p21, and p53 mRNA) were found to be existing in Beijing Cancer Institute L1210 and two different cloned cell strains. Expression of c myc, c fos, p53 mRNA could distinguish L3E11 and L3F9 cloned cells.

关 键 词：L1210 克隆细胞 原位杂交 分子探针 

分 类 号：R730.2[医药卫生—肿瘤]