人β-防御素基因在女性生殖道及妊娠相关组织中的表达  

The Humanβ-defensins Expression in Female Genital Tract and Pregnancy-related Tissues

作　　者：冯艳[1] 潘小玲[2] 黄宁[1] 冯云[1] 吴琦[1] 王伯瑶[1] 

机构地区：[1]四川大学华西基础医学与法医学院感染免疫研究室,成都610041 [2]四川大学华西第二医院妇产科

出　　处：《四川大学学报（医学版）》2003年第2期217-219,共3页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号 3 980 0 14 6);纽约中华医学会基金资助

摘　　要：目的　检测人β-防御素 (HBDs)基因在女性生殖道各段组织及妊娠相关组织中的表达。方法　采用逆转录聚合酶链反应法 (RT- PCR)检测正常女性生殖道各段组织及怀孕妇女妊娠相关组织中 HBD- 1、HBD- 2的表达情况 ,用内参照β- actin的特异引物作 RT- PCR。结果　细胞株 ME- 180表达 HBD- 1m RNA,而不表达 HBD- 2m RNA;正常女性阴道、子宫颈、子宫内膜、输卵管和卵巢 5种组织中均发现有 HBD- 1m RNA的表达 ,除阴道、卵巢外的其余组织中也有 HBD- 2 m RNA的表达 ;绒毛膜、绒毛、羊膜、胎盘和脐带 5种妊娠相关组织中 ,HBD- 1m RNA在除羊膜外的各组织中均有表达 ,而 HBD- 2 m RNA则在绒毛膜、绒毛及胎盘组织中表达。结论　 HBDs在正常妇女生殖道及妊娠相关组织中广泛表达 ,提示其在维系正常妇女及孕妇生殖道内环境稳定。Objective Human β defensins(HBDs) are small, cationic endogenous antimicrobial peptides isolated from the hemodialates of patients with chronic renal failure (HBD 1) and Psoriasic skin (HBD 2). They may play an important role in mucosal innate host defense against infections. In this study, we examined their mRNAs expression in normal and pregnant women genital tract. Methods Reverse transcription polymerase chain reaction (RT PCR) was performed in the detection of HBD 1 and HBD 2 mRNA expression in the samples from normal and pregnant women genital tract. β actin was taken as positive control. Results RT PCR detection revealed that HBD 1 mRNA expressed in all samples of normal wemen taken from vagina, cervix, endometrium, fallopian tube, and ovary, and HBD 2 mRNA widely expressed in genital tract except vagina and ovary. In pregnant women, HBD 1 mRNA widely expressed in chorion,villus, placenta, and umbilical cord, but not in amnion, and HBD 2 mRNA were detected in chorion,villus and placenta tissues. Conclusion The findings of this study on the wide expression of HBD 1 and HBD 2 in both the normal and the pregnant women genital tract suggest that HBDs may play an important role in human genital tract defense mechanisms.

关 键 词：抗菌肽 人β-防御素 女性生殖道 

分 类 号：R363[医药卫生—病理学]