p57^(kip2)蛋白和增殖细胞核抗原在胰腺癌组织中的表达及其临床意义探讨  

Expression of p57^(kip2)and PCNA protein in human pancreatic cancer and its clinical significances

作　　者：岳辉[1] 于皆平[2] 

机构地区：[1]沈阳军区总医院,110016 [2]武汉大学人民医院消化内科,430060

出　　处：《中国实用内科杂志》2003年第4期229-230,共2页Chinese Journal of Practical Internal Medicine

基　　金：辽宁省博士启动基金资助 (编号 :2 0 0 2 10 2 9)

摘　　要：目的　探讨细胞周期调控抑制因子 p5 7kip2 蛋白和增殖细胞核抗原 (PCNA)表达在胰腺癌发生发展中的作用及其临床意义。方法　采用免疫组织化学技术SP法 ,检测p5 7kip2 和PC NA在 32例胰腺癌组织和癌旁胰腺组织中的表达情况。结果　p5 7kip2 蛋白阳性表达率在胰腺癌组织中为 46 9% ,显著低于癌旁胰腺组织 (P <0 0 5 ) ,并与胰腺癌组织分化程度有关 (P <0 0 5 )而与淋巴结转移无关 ( χ2 =3 698,P >0 0 5 ) ;PCNA蛋白阳性表达率在胰腺癌组织中为71 9% ,显著高于癌旁胰腺组织 (P <0 0 5 ) ,并与胰腺癌组织分化程度和淋巴结转移均有关 (P<0 0 5 )。结论　p5 7kip2 、PCNA可能与胰腺癌发生、发展密切相关 ;p5 7kip2 蛋白表达降低、PCNA蛋白过度表达有助于胰腺癌的发生及细胞分化程度及预后的判定 ,但 p5 7kip2Objective To investigate the effects of p57 kip2 protein PCNA on the occurrence and progression of human pancreatic cancer. Methods The expression of p57 kip2 , PCNA in tumor tissues and adjacent tissues of 32 patients with pancreatic cancer were detected by SP immunohistochemical technique. Results p57 kip2 protein positive-expression rate in tumor tissue of pancreatic cancer was 46 9% , which was significantly lower than that in adjacent pancreatic tissues (P<0 05), p57 kip2 protein positive-expression correlated significantly with tumor cell differentiation (P<0 05), but did not correlate with lymph node metastasis(χ 2 =3 698,P>0 05); PCNA positive-expression rate in tumor tissues was 71 9%, which was significantly higher than that in adjacent pancreatic tissues (P<0 05), PCNA positive-expression correlated significantly with tumor cell differentiation and lymph node metastasis (χ 2 =7 513,P<0 05). Conclusions p57 kip2 , PCNA may play an important role in genesis and progress of pancreatic cancer. The decreased expression of p57 kip2 protein,over-expression of PCNA protein contribute to genesis of pancreatic cancer and determination of cell differentiation and prognosis of the diseases,but the decreased expression of p57 kip2 does not correlate with lymph node metastasis of pancreatic cancer.

关 键 词：P57^KIP2蛋白 增殖细胞核抗原 胰腺癌 免疫组织化学技术 预后 

分 类 号：R735.9[医药卫生—肿瘤]