混合菌种在发酵法生物产氢中的协同作用  

Cooperation of Mixed Culturing Bacteria in the Hydrogen Production by Fermentation

作　　者：林明[1] 任南琪[1] 王爱杰[1] 王相晶[1] 

机构地区：[1]哈尔滨工业大学市政与环境工程学院,哈尔滨150090

出　　处：《环境科学》2003年第2期54-59,共6页Environmental Science

基　　金：国家"973"项目 (G2 0 0 0 0 2 6 4 0 2 ) ;国家"86 3"项目(2 0 0 1AA5 15 0 30 )

摘　　要：为探讨产氢发酵细菌混合培养时菌种间的协同作用 ,本研究在间歇试验条件下 ,分别考察以葡萄糖和复杂有机物 (淀粉、牛肉膏、聚乙二醇乙二酸酯和胰蛋白胨 )为底物时 ,5株HPB (B49、H1、LM1 2、LM1 1和B5 1 )混合培养 ,高效HPB(B49)与 3株非产氢发酵细菌 (L1 0、拟 3 2和芽孢 1 )分别混合培养 ,以及B49与活性污泥混合培养对产氢能力的影响 .试验结果表明 ,混合菌种间协同作用发挥是有条件的 .当利用葡萄糖发酵产氢时 ,菌种间对共同底物的竞争使其协同作用无法发挥 ,从而制约了高效产氢细菌 (HPB)的产氢能力 ;而利用复杂有机物发酵产氢时 ,菌种间的协同作用得以发挥 ,并促进了高效HPB产氢能力的提高 .同时提出 。In order to discuss the cooperation of H 2 producing fermentation bacteria (HPFB) in mixed culture, a batch test fed with glucose and complex organic substance (starch, beef, PEP and peptone) respectively, was conducted to investigate the effects of mixed culture on H 2 producing ability. For the systemic and accurate analysis, three kinds of the mixed culture were use to this batch test. It included that the mixed culture with five strains of HPFB (B49, H1, LM12, LM11 and B51), B49 and three stains of non HPFB (L10, Bacteroide3 2, Sporobacter1), and B49 and hydrogen producing sludge. The results showed that the cooperation of mixed culturing bacteria was conditional on the substrates. When fed with glucose, which were easily utilized by HPB, the H 2 producing ability of HPB was restrained because of the competition on the co substrate between HPB and other fermentation bacteria, and it was quite difficult for the cooperation of mixed culturing bacteria to be performed. When fed with complex organic substance, the H 2 producing ability of HPB was enhanced via the cooperation of mixed culturing bacteria. Furthermore, a strategy was put forward,that is, different kind of HPB cultures could be adopted according to the difference of substrates.

关 键 词：产氢 发酵细菌 混合培养 协同作用 

分 类 号：X172[环境科学与工程—环境科学]