rhBMP-2m对化疗损伤小鼠的修复治疗作用  

Reparative and therapeutic effect of rhBMP-2m on mice injured by chemotherapy

作　　者：刘斌[1] 袁诚君[2] 田琼[1] 薛采芳[3] 

机构地区：[1]第四军医大学基础医学教学实验中心,陕西西安710032 [2]西安交通大学医学院免疫学教研室 [3]第四军医大学病原生物学教研室

出　　处：《细胞与分子免疫学杂志》2003年第2期150-152,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No . 3 9970 2 3 1 )

摘　　要：目的 :探讨重组人骨形成蛋白 2 (rhBMP 2m)对环磷酰胺 (CTX)致骨髓损伤小鼠的治疗作用。方法 :18只小鼠随机分为 3组 :即CTX注射组、BMP治疗组和PBS对照组 ,每组 6只小鼠。CTX组和BMP治疗组小鼠 ,一次性腹腔注射 2 0 0mg/kgCTX建立骨髓损伤的模型。注射CTX第 2天 ,BMP治疗组每只小鼠腹腔注射 0 .5mg的rhBMP 2m开始治疗。观察 3组小鼠外周血白细胞数的变化。分别在第 5天和第 8天 ,用流式细胞仪 (FCM )检测骨髓有核细胞中DNA的含量及细胞周期的变化 ,同时进行粒单细胞集落形成 (CFU GM)细胞培养。结果 :注射CTX后第 4天 ,BMP治疗组和CTX注射组的外周血白细胞数均降至最低点 ,然后逐渐回升 ,两组相比较无显著差异 (P >0 .0 5 )。注射CTX后第 5天 ,BMP治疗组骨髓有核细胞中 ,G0 /G1期细胞的比例较CTX组明显提高 ,细胞的坏死及凋亡率明显下降 (P <0 .0 1)。第 8天 ,BMP治疗组的骨髓有核细胞数较CTX组增加明显 (P <0 .0 1)。结论AIM: To probe the therapeutic effect of recombinant human bone marrow morphogenetic protein 2 maturation peptide(rhBMP 2m) on mouse bone marrow injury caused by cyclophosphamide (CTX). METHODS: 18 mice were divided randomly into 3 groups, namely CTX injection group(CTX group), BMP therapy group(BMP group) and PBS control group(Control group), 6 mice each group. CTX of 200 mg/kg per mouse was intraperitonealy(IP) injected at a time to BMP group and CTX group so as to establish the experimental model of mouse bone marrow injury. In BMP group, the therapy started from the second day after injection of CTX by using IP injection of 0.5 mg BMP per mouse each day. In control group, PBS was injected only. Changes of peripheral blood leukocyte numbers in 3 groups were observed . On the 5th and 8th day after CTX injection, DNA content in mouse bone marrow karyocytes and variation of cell cycle were analysed by flow cytometry(FCM). Colony forming unit granulocyte macrophage(CFU GM) was cultivated simultaneously. RESULTS: On the 4th day after injection of CTX, the leukocyte number in mouse peripheral blood of CTX group dropped to the lowest level, and then picking up gradually. In respect to the variation of the leukocyte number, there was no significant difference between BMP group and CTX group ( P >0.05). On the 5th day, the ratio of G 0/G 1 phase cells in BMP group was notably higher than that of CTX group,and the necrotic and apoptotic rates decreased markedly ( P <0.01). On the 8th day, the number of karyocytes in mouse bone marrow of BMP group was obviously more than that of CTX group ( P <0.01). CONCLUSION: BMP has some therapeutic effect on mouse bone marrow injury caused by CTX.

关 键 词：重组人骨形成蛋白-2 化学疗法 环磷酰胺 骨髓损伤 造血干细胞 动物实验 

分 类 号：R730.53[医药卫生—肿瘤] R551.3[医药卫生—临床医学]