应用微卫星图谱定位稻米性状的QTL  

Identification of QTLs underlying grain traits in rice using SSLP linkage map

作　　者：汪斌[1] 兰涛[2] 吴为人[2] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学作物科学学院,福建福州350002

出　　处：《福建农业学报》2003年第1期11-15,共5页Fujian Journal of Agricultural Sciences

基　　金：国家863计划项目(Z16-02-03)。

摘　　要：以两系杂交稻强优组合培矮64s/E32的1个加倍单倍体(doubled haploid,DH)群体(含有86个株系)为供试材料,利用福建农林大学遗传工程实验室已构建的微卫星标记连锁图,应用复合区间作图法对控制水稻糙米性状(粒长、粒宽和粒重)的QTL进行定位。在本试验中共定位了18个QTL,其中8个控制粒长,4个控制粒宽,6个控制粒重,它们对粒长、粒宽和粒重表型变异的贡献率分别为78.61％、68.62％和72.06％。Quantitative trait loci (QTLs) underlying brown rice length (BRL) , brown rice breadth (BRB) and brown rice weight (BRW) were mapped based on a constructed SSLP linkage map by using a doubled haploid population including 86 lines. QTL mapping was conducted with the whole genome map using the method of composite interval mapping (CIM). Eighteen QTLs were detected, 8 for BRL, 4 for BRB and 6 for BRW, which could explain 78.61%, 68.62% and 72.06% phenotypic variances of BRL, BRB and BRW, respectively. The location of several QTLs, particularly those with larger effects, are consistent with previous reports of other researchers.

关 键 词：稻米 数量性状基因座 QTL 微卫星图谱 基因定位 复合区间作图法 品质性状 

分 类 号：S511[农业科学—作物学]