左旋多巴对帕金森病大鼠血清兴奋性氨基酸及抗氧化指标的影响  

Effects of levodopa on the serum excitatory amino acids and anti-oxidative indexes in rats with Parkinsonism

作　　者：肖勤[1] 翁中芳[1] 张璟[1] 梁樑[1] 徐洁懿[1] 陈生弟[1] 

机构地区：[1]上海第二医科大学附属瑞金医院神经科帕金森病诊疗与研究中心,200025

出　　处：《临床神经病学杂志》2003年第2期69-71,共3页Journal of Clinical Neurology

基　　金：国家重点基础研究规划"脑功能和脑重大疾病的基础研究"项目 (G1 9990 540 0 8);国家科委"九五"攻关项目 (96 90 6 0 5 0 8);卫生部科学研究基金 (98 1 31 9);上海市卫生系统百名跨世纪优秀学科带头人培养计划 (97BR0 0 1 )资助项目

摘　　要：目的　研究左旋多巴对帕金森病 (PD)大鼠血清兴奋性氨基酸含量及部分抗氧化指标的影响。方法　应用 6 羟基多巴胺 (6 OHDA)制备部分和严重损毁的PD大鼠模型 ,给两种模型大鼠连续服用不同剂量左旋多巴 /苄丝肼 3个月 ,利用高压液相色谱 紫外检测仪测定大鼠血清谷氨酸、谷氨酰胺和天门冬氨酸的含量 ,采用生化法测定PD大鼠血清超氧化物歧化酶、丙二醛、谷胱甘肽 (GSH)及谷胱甘肽过氧化物酶的水平。结果　左旋多巴可使严重损毁组大鼠的血清天门冬氨酸水平明显升高 (P <0 .0 5 ) ,而谷氨酸、谷氨酰胺水平无明显改变。使用左旋多巴后血清超氧化物歧化酶、丙二醛及谷胱甘肽水平无明显改变 ,而小剂量左旋多巴组大鼠血清谷胱甘肽过氧化物酶水平显著升高 (P <0 .0 1)。结论　长期使用左旋多巴对PD模型大鼠可能有兴奋毒性作用 。Objective To study the effects of levodopa on the serum excitatory amino acids and some anti oxidative indexes in rats with parkinsonism.Methods Different doses of levodopa/benserazide were treated orally for three months in rats with 6 OHDA induced partial or severe injuries of unilateral nigrostriatal pathway. The levels of serum aspartic acid, glutamate and glutamine were measured by high performance liquid chromatography(HPLC). Serum superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GSH PX) were detected.Results After the rats were treated with levodopa,the concentration of serum aspartic acid was significantly increased in the severely 6 OHDA lesioned rats ( P <0.05), while the contents of glutamate and glutamine remained unchanged. The serum glutathione peroxidase activities in lower dose group increased significantly ( P <0.01), but there was no obvious change on serum levels of superoxide dismutase, malondialdehyde and glutathione.Conclusion It is demonstrated that long term use of levodopa might be excitatory toxic to PD rats, and levodopa in lower dose might facilitate the anti oxidative GSH system.

关 键 词：左旋多巴 帕金森病 PD 血清 兴奋性氨基酸 抗氧化指标 谷氨酸 

分 类 号：R742.5[医药卫生—神经病学与精神病学]