产氢发酵细菌培养基的选择和改进  

Selection and improvement of culture for hydrogen producing bacteria

作　　者：林明[1] 任南琪[1] 马汐平[2] 王爱杰[1] 马放[1] 王相晶[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150090 [2]辽宁大学环境科学系,辽宁沈阳110036

出　　处：《哈尔滨工业大学学报》2003年第4期398-402,共5页Journal of Harbin Institute of Technology

基　　金：国家重点基础研究发展规划资助项目(G2 0 0 0 0 2 64 0 2 )

摘　　要：高效产氢菌株的筛选与分离是生物制氢技术应用的重要基础之一 ,现有培养基都存在一定缺陷 .为分离到高效产氢菌株 ,提出LM系列培养基配方 ,并通过产氢发酵细菌的分离和生长实验 ,确定了培养基的强还原剂为半胱氨酸 ,pH值为 6 .根据培养基对厌氧菌的分离数量、种类和培养基成分的分析 ,选择LM - 1培养基为产氢发酵细菌的分离培养基 .LM - 1培养基分离出的 5株高效产氢发酵细菌属于 4个菌属 ,其中拟杆菌属、克雷伯氏菌属是国际上尚未分离到的高效产氢发酵细菌 .利用LM - 1培养基进行高效产氢细菌分离操作 。LM series cultures were proposed and it was determined through bacteria isolation and experimental growth that the reducer was L-cysteine and the value of pH was 6. The number and kinds of bacteria during LM-1culture batch culturing justified the use of LM-1culture for isolation of hydrogen producing fermentation bacteria. The same conclusion was drawn from the analysis of the culture's composition. 5 isolated stains of high hydrogen producing ferment bacteria were identified as belonging to 4 genera. Among these genera, Bacteroides and Klebsiella were not belonging to the familiar genus of isolated high hydrogen producing fermentation bacteria in the world. LM-1 culture was for isolation of high effective hydrogen production bacteria, and proved highly effective.

关 键 词：发酵细菌 培养基 高效产氢菌株 生物制氢 氢能 

分 类 号：TQ116.2[化学工程—无机化工] TQ929[轻工技术与工程—发酵工程]