光纤倏逝波生物传感器及其应用  

Fiber-Optic Evanescent Wave Biosensor and Its Application

作　　者：黄惠杰[1] 翟俊辉[2] 任冰强[1] 杨瑞馥[2] 赵永凯[1] 程兆谷[1] 杜龙龙[1] 路敦武[1] 

机构地区：[1]中国科学院上海光学精密机械研究所,上海201800 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《光学学报》2003年第4期451-454,共4页Acta Optica Sinica

摘　　要：以光纤倏逝波原理 ,研制成功一台光纤倏逝波生物传感器。该传感器对纯净的Cy5荧光染料溶液的检测灵敏度达 0 .0 1μmol/l;对浓度为 0 .0 1μmol/l的嗜肺军团菌核酸样品的测量信噪比为 4.6 1。所有检测结果与商品化生物芯片扫描仪同时检测得到的结果具有很好的一致性。A fiber-optic evanescent wave biosensor has been developed based on the principle of evanescent wave while light propagates in optical fiber. Sensitivity limit of 0.01 nmol/l is obtained from the detection of Cy5 serial solutions with various concentrations. And an ideal result of signal-to-noise ratio of 4.61 is obtained when the biosensor is used to measure Legionella pneumophila solution of 0.01 μmol/l. All the results are comparable with that obtained by a commercial biochip scanner.

关 键 词：光纤倏逝波生物传感器 性能检测 荧光染料溶液 嗜肺军团菌核酸 信噪比 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] Q63[自动化与计算机技术—控制科学与工程]