养殖褐牙鲆细胞色素b基因序列的初步研究  

Sequences of mitochondrial cytochrome b gene of reared Paralichthys olivaceus

作　　者：高天翔[1] 张秀梅[2] 朱杰[2] 柳广东[2] 张亚平[1] 

机构地区：[1]中国科学院昆明动物研究所细胞与分子进化开放研究实验室,云南昆明650223 [2]中国海洋大学水产学院,山东青岛266003

出　　处：《中国水产科学》2003年第2期93-96,共4页Journal of Fishery Sciences of China

基　　金：国家自然科学基金项目资助(300705930).

摘　　要：对养殖褐牙鲆(Paralichthysolivaceus)的线粒体DNACytb基因的部分序列进行测定,测得的目的DNA片段的长度为410bp,其A(104bp)、T(119bp)、C(117bp)、G(70bp)4种碱基平均含量分别为25.4%、29.0%、28.5%、17.1%。在28个褐牙鲆个体中共出现了3种单倍型。白化褐牙鲆出现的第1种和第3种单倍型个体数分别为10尾(占白化褐牙鲆样本数的90.91%)和1尾(9.09%);6尾黑化褐牙鲆均出现第1种单倍型(100%);正常褐牙鲆出现的3种单倍型尾数分别为7尾(占正常褐牙鲆样本数的55.56%)、2尾(22.22%)和2尾(22.22%);测得的序列与既知序列间在第6bp、第19bp和第402bp碱基处出现差异。由于褐牙鲆Cytb基因的高度同源性,研究其白化、黑化和正常状态时出现的序列差异,对于寻找褐牙鲆白化机理研究的分子标记意义重大。Twentyeight samples of reared Japanese flounder Paralichthys olivaceus were used. Among them, 11 were with albinism, 6 with hypermelanosis and the other 11 were at normal state. The partial sequences of their mitochondrial DNA cytochrome b gene were sequenced and analyzed. The sequence length of the target fragments was 410 bp and the contents of A(104 bp) , T(119 bp), C(117 bp) and G(70 bp) in the sequence were 25.4%, 29.0%, 28.5% and 17.1%, respectively. Three types of haplotype were observed in the 28 individuals that among the 11 albinotic fish, the first type of haplotype presents in 10 individuals, which makes up 90.91% of the 11, and the third type of haplotype presents in one individual, which makes up 9.09% of the 11. Also, the first type of haplotype exists in all of the 6 melanic fish. But among the 11 normal fish, the first type of haplotype can be observed in 7 individuals (55.56% of normal fish in number), and the second type and the third type of haplotype can be observed in two and two individuals, respectively. Between the sequences obtained and those from Genbank, three sites were different at the positions of 6 bp, 19 bp and 402 bp, respectively. The purpose of this study is to find the molecular marker for the study of albinotic machanism in P. olivaceus.

关 键 词：褐牙鲆 细胞色素B 基因序列 线粒体DNA 单倍型 白化机理 分子标记 

分 类 号：Q959.486[生物学—动物学] S965.399[农业科学—水产养殖]