人骨髓间充质干细胞表达多种造血细胞因子  

Human Bone Marrow Mesenchymal Stem Cells Express Multiple Hemato-poietic Growth Factors

作　　者：朱光荣[1] 周小玉[1] 陆化[1] 周建伟[1] 李爱萍[1] 徐卫[1] 李建勇[1] 汪承亚[1] 

机构地区：[1]南京医科大学第一附属医院血液科,南京210029

出　　处：《中国实验血液学杂志》2003年第2期115-119,共5页Journal of Experimental Hematology

基　　金：江苏省卫生厅重点课题基金项目资助(编号H2 0 0 13 1);南京医科大学创新基金资助项目(编号CX2 0 0 10 0 4)

摘　　要：为了研究培养的人骨髓间充质干细胞在造血中的生物作用 ,采用RT PCR方法在mRNA水平上分析体外培养的人骨髓间充质干细胞 (BM MSC)造血因子的表达 ,以及氢化考的松对BM MSC表达造血细胞因子的影响。结果显示 ,体外传代培养的正常人、白血病和淋巴瘤等血液病人BM MSC均能表达SCF ,Flt3 ligandTPO ,LIF ,IL 6和IL 11等重要的造血细胞因子 ,但不表达G CSF ,GM CSF和IL 3。不同代数的BM MSC细胞 ,造血细胞因子的表达相同。BM MSC经氢化考的松作用 7- 2 1天后 ,可诱导G CSFmRNA表达 ,但不诱导GM CSF的表达 ,细胞在形态学上也未发生明显改变。结论 :体外培养的正常人和本组血液病病人BMTo study the biological role of human cultrued bone marrow mesenchymal stem cell (BM MSC) in hematopoiesis by investigation of its expression of multiple hematopoietic growth factors, RT PCR was used to analyze the expression of SCF, Flt3 ligand, TPO, LIF, G CSF, GM CSF, IL 3, IL 6 and IL 11 at mRNA level for human BM MSC from healthy donors and patients with leukemia and lymphoma. BM MSC were incubated with or without hydrocortison (HC). The results clearly showed that the cultured BM MSC expressed mRNA of SCF, Flt3 ligand, TPO, LIF, IL 6 and IL 11 at passages 3 up to 15, but did not express G CSF, GM CSF and IL 3. The same expression pattern of above cytokines was seen also for the patient′s BM MSC. HC was able to induce BM MSC to express G CSF but not to express GM CSF. BM MSC seemed not to change morphologically after incubation with HC for up to 21 days. In conclusion, both normal and patient BM MSC should be potential to promote hematopoiesis according to their expression of multiple hematopoietic cytokines, and HC is able to induce hematopoietic growth factor expression.

关 键 词：人骨髓间充质干细胞 造血细胞因子 表达 生物作用 氢化考的松 白血病 淋巴瘤 

分 类 号：R733[医药卫生—肿瘤] R331.22[医药卫生—临床医学]