微囊化转mIFN-γ基因CHO细胞皮下移植促进荷瘤小鼠T_H1T_H2的漂移  

Influence of microencapsulated CHO cells translated mIFN-γ on T_H1/T_H2 in tumor burden mice

作　　者：潘月龙[1] 郑树[1] 孝作祥[1] 姚航平[2] 

机构地区：[1]浙江大学肿瘤研究所 [2]浙江大学免疫学研究所,杭州310009

出　　处：《中华微生物学和免疫学杂志》2003年第3期195-198,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的　通过观察微囊化转mIFN γ基因CHO细胞皮下移植对荷瘤小鼠TH1 TH2类细胞因子的影响 ,进一步了解外源性IFN γ对TH1 TH2平衡的调节作用 ,同时验证微囊化基因工程细胞移植治疗恶性肿瘤的可行性。方法　将mIFN γ基因转染到正常细胞CHO ,然后进行微囊化 ,移植到荷瘤小鼠的皮下 ,2周后 ,测定小鼠血清TH1和TH2类细胞因子IFN γ、IL 2、IL 12、IL 4、IL 10的水平 ,并与对照组相比较。同时测量肿瘤的平均直径 ,以观察肿瘤的生长速度 ,并观察荷瘤小鼠的生存期。结果　经微囊化转mIFN γ基因CHO细胞皮下移植干预治疗后 ,小鼠血清TH1类细胞因子IFN γ、IL 2、IL 12水平明显升高 ,而TH2类细胞因子IL 4、IL 10变化不明显 ;小鼠肿瘤的生长速度明显减慢 ,生存期明显延长。结论　外源性IFN γ促进荷瘤小鼠TH1细胞的分化 ,使TH1 TH2向着TH1方向漂移 ;微囊化基因工程细胞的移植 ,有望替代基因工程药物 ,成为治疗恶性肿瘤的又一新平台。Objective To study the influence of transplantation of microencapsulated CHO cells translated IFN-γ gene on T H1/T H2 type cytokines, and adjustment of foreign IFN-γ to T H1/T H2 cells balance. The feasibility of microencapsulated genetic cells as tumor-killer was studied. Methods The mIFN-γ gene was translated into CHO cells. After microencapsulated, the CHO cells translated IFN-γ gene were transplanted into tumor burden mice. After 14 days, the level of serum IFN-γ, IL-2, IL-12, IL-4, IL-10 was examined. The tumor size and survival time was studied also. Results The level of serum IFN-γ, IL-2, IL-12 was significantly higher, but the change of IL-4, IL-10 were not significantly. The speed of tumor growth was significantly slowed down. The survival time was elongated. Conclusions The foreign IFN-γ promotes T H1 cells differentiation, T H1/T H2 move to T H1. The microencapsulated genetic enginnering cells may be a new candidate for cancer treatment as an alternative of genetic enginnering drugs. [

关 键 词：微囊 IFN-Γ TH1/TH2 肿瘤 基因工程 免疫学 

分 类 号：R730.3[医药卫生—肿瘤]