脑梗死大鼠康复训练后脑功能恢复及免疫组织化学改变  

Cerebral functions and immunohistochemical changes caused by rehabilitation in rats after infarction

作　　者：唐强[1] 秦颖[2] 倪金霞[1] 王德生 

机构地区：[1]黑龙江中医药大学附属第二医院康复医学科,黑龙江哈尔滨市150001 [2]哈尔滨医科大学第一临床医学院干部病房,黑龙江哈尔滨市150001

出　　处：《中国康复理论与实践》2003年第3期136-138,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：中国博士后科学基金 (中博基 [2 0 0 0 ] 2 3号 )

摘　　要：目的研究康复对急性脑梗死大鼠脑功能恢复的作用机制。方法采用抽签法将 3 0只雄性Wister大鼠随机分为 3组 :A组 (假手术组 )、B组 (造模组 )、C组 (康复组 ) ;运用血管内栓线法制备脑缺血动物模型 ;术后 2 4h、3d及 7d分别进行Bederson神经功能评分 ,平衡木、转棒、网屏测评 ,并于术后第 7天测定脑组织内神经生长因子 (NGF)、脑源性神经生长因子 (BDNF)、转化生长因子 (NGF)、碱性成纤维细胞生长因子 (bFGF)。结果A组大鼠Bederson神经功能评分无改变 ,其他两组在术后 2 4h出现评分升高 ,随时间延长呈逐渐恢复趋势 ,但各时间点与A组均有高度显著性差异 (P <0 0 1) ;各组大鼠术后 2 4h均出现抓握、行走及协调能力障碍 ,组间两两比较 ,无显著性差异 (P >0 0 5 ) ;随着时间的延长 ,A组的各项功能很快恢复 ,B、C组也有一定程度的改善 ,但远不及A组 ,差异显著 (P <0 0 1) ;与B组比较 ,缺血后 3d ,C组平衡木试验有显著性差异 (P <0 0 5 ) ;缺血后 7d ,除Berderson评分外 ,均有显著性差异。A组与B组术后 7d皮层及海马部位均有少量的NGF、BDNF、TGF及bFGF表达 ,两组间比较无显著性差异 (P >0 0 5) ,C组NGF、BDNF、TGF及bFGF表达明显升高 (P <0 0 1)。结论康复能提高脑梗死大鼠平衡、行走及抓握能力 ,增加?ObjectiveTo study the rehabilitation mechanism of acute cerebral infarction.Methods30 male Wistar rats were randomly divided into 3 groups:group A(sham group),group B(model group) and group C(rehabilitation group).Animal model was made by the middle cerebral artery occlusion(MCAO).For each group,Bederson Neural Function was scored and balancing wood,rotating bar,and net screen were tested24 hours,3 days and 7 days after operation respectively.The nerve growth factor(NGF),brain derived neurotrophic factor(BDNF),transforming growth factor(TGF),basic fibroblast growth factor(bFGF)in brain tissues were tested.ResultsBederson neural function scores of group A had no changes,while those of the other two groups increased after 24 hours and had a tendency to restore gradually with time continuing,but indicated significant differences at each time,compared with group A( P <0 01).The abilities of grasping ,walking and coordinating of all the groups after 24 hours by operation handicapped,and there was no difference among the groups( P >0 05);with time continuing,every function of group A restored soon and that of group B or C improved,to some degree,but showed significant difference to group A( P <0 01).After 3 days by operation,balancing wood test of group C was better than that of group B( P <0 05);after 7 days,except Bederson neural function score,there was difference between the group C and B,that is,the former was superior to the latter. After 7 days by operation,there were a few NGF,BDNF,TGF and bFGF expressions in hippocampus and cortex areas of group A and B,and there was no difference between this two groups( P >0 05),while those of group C greatly increased,compared with group A and B( P <0 05-0 01).ConclusionsRehabilitation therapy can improve the MCAO rats' ability of equilibrating,grasping and walking and strengthen the myodynamia,as well as induce the expression of NGF,BDNF,bFGF and TGF in cortex and hippocampus.

关 键 词：免疫组织化学 脑梗死 康复 神经营养因子 大鼠 

分 类 号：R743.33[医药卫生—神经病学与精神病学]